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| Effect and Novel Mechanism of IL-17A on Apoptosis of HepG2 Cell |
| YIN Wen-jun, LI Mian-li, WANG Qiu-ping, et al |
| Department of Clinical Laboratory, the First Affiliated Hospital to University of South China, Hengyang, Hunan 421001, China |
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Abstract 【Objective】To explore the effects of interleukin-17A (IL-17A) on apoptosis of HepG2 cell and investigate the novel mechanism. 【Methods】Quantitative real-time PCR (qRT-PCR) and Western blot were used to detect IL-17A expression in normal hepatocytes (L-02) and hepatic cancer cell lines (Huh7, HepG2 and PLC/PRF/5). Subsequently, HepG2 cells were selected and treated with recombinant IL-17A (rIL-17A) and/or myeloid cell leukemia-1 (Mcl-1) siRNA. Apoptotic rate was analyzed by flow cytometry. The expression of Mcl-1 was measured by qRT-PCR and Western Blot. 【Results】 Compared to those in L-02 cells, the mRNA and protein levels of IL-17A were significantly increased in Huh7, HepG2 and PLC/PRF/5 cells, and HepG2 cells had the highest expression (P<0.01). Administration of rIL-17A reduced apoptotic rate and increased Mcl-1 expression in HepG2 cells (P<0.01). However, siRNA-mediated knockdown of Mcl-1 reversed the inhibitory effect of rIL-17A on HepG2 cell apoptosis (P<0.05).【Conclusion】IL-17A is highly expressed in hepatic cancer cells. IL-17A reduces HepG2 cell apoptosis by upregulating Mcl-1 expression.
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Received: 10 May 2018
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2018, Vol. 35 
