磁微粒化学发光法与梅毒螺旋体颗粒凝集实验联合检测在梅毒诊断中的应用价值

doi:10.3969/j.issn.1671-7171.2025.06.026

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Abstract 【Objective】 To explore the diagnostic value of combining chemiluminescent microparticle immunoassay (CMIA) and Treponema pallidum particle agglutination assay (TPPA) in the diagnosis of syphilis.【Methods】 Venous blood samples were collected from 40 clinically confirmed syphilis patients and 40 healthy individuals. All samples were tested using both CMIA and TPPA. The results of the two methods were compared. Subjects were also grouped based on CMIA derived S/CO values, and TPPA results were compared across these groups.【Results】Among the 80 subjects, CMIA detected 41 positives and 39 negatives; TPPA detected 38 positives and 42 negatives. There was no statistically significant difference between the results of CMIA and TPPA (P>0.05). The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of CMIA were 100.00%, 97.50%, 97.56%, 100.00%, and 98.75%, respectively, with a Kappa value of 0.975. Compared with clinical diagnosis, there was no significant difference (P>0.05). The corresponding values for TPPA were 95.00%, 100.00%, 100.00%, 95.24%, and 97.50%, respectively, with a Kappa value of 0.950, also showing no significant difference from clinical diagnosis (P>0.05). The concordance rate between CMIA and TPPA was 96.25%, with a Kappa value of 0.925 (P>0.05). TPPA positivity increased with higher S/CO values. When S/CO > 7, TPPA positivity reached 100.00%.【Conclusion】 Both CMIA and TPPA exhibit high sensitivity, specificity, and accuracy in syphilis diagnosis. TPPA positivity increases significantly with higher S/CO values. A stratified diagnostic approach based on an S/CO threshold suggests that high S/CO(>7) cases can be directly diagnosed following CMIA screening without TPPA confirmation, thereby reducing detection time and cost. TPPA verification remains necessary for cases with S/CO ≤ 7. This optimized diagnostic workflow can improve efficiency and is well-suited for large-scale screening programs.

Key words： Syphilis Luminescent Measurements Syphilis Serodiagnosis

Received: 10 March 2025

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R759.1

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	HE Zhe
	ZHANG Limei
	LIU Chengguo

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HE Zhe,ZHANG Limei,LIU Chengguo. Application Value of Combined Detection of Chemiluminescent Microparticle Immunoassay and Treponema Pallidum Particle Agglutination Test in the Diagnosis of Syphilis[J]. JOURNAL OF CLINICAL RESEARCH, 2025, 42(6): 1004-1006.

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http://journal07.magtech.org.cn/yxlcyj/EN/10.3969/j.issn.1671-7171.2025.06.026 OR http://journal07.magtech.org.cn/yxlcyj/EN/Y2025/V42/I6/1004

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