miR-181a-5p介导虎杖苷体外诱导乳腺癌MDA-MB-231细胞凋亡的机制研究

doi:10.3969/j.issn.1671-7171.2022.07.015

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Abstract 【Objective】 To investigate the effect of polydatin on the proliferation and apoptosis of MDA-MB-231 cells in breast cancer. 【Methods】 Breast cancer MDA-MB-231 cells was treated at different concentrations (0, 2, 4, 6, 8, 16 μmol/L) of polydatin; Cell proliferation was detected by MTT method. Breast cancer MDA-MB-231 cells were divided into control group (routine culture) and polydatin group(6 μmol/L polydatin treatment), polydatin +Anti-NC group (transfection inhibitor control, 6 μmol/L polydatin treatment), polydatin+Anti-miR-181a-5 group (transfected with miR-181a-5 inhibitor, 6 μmol/L polydatin treatment). The apoptosis of MDA-MB-231 cells was detected by flow cytometry, and the expression levels of Bax, c-caspase-3 and caspase-3 proteins in MDA-MB-231 cells were detected by Western blot; The expression level of mir-181a-5p in MDA-MB-231 cells was measured by realtime PCR. 【Results】 Compared with the control group, 2, 4, 6, 8, 16 μmol/L,the absorbance value of cells treated with polydatin decreased significantly (all P<0.05).Compared with polydatin+Anti-NC group, the level of miR-181a-5p in polydatin+Anti-miR-181a-5 group decreased, the absorbance value increased, the apoptosis rate decreased, and the levels of Bax, c-caspase-3, caspase-3 protein in breast cancer cells decreased (P<0.05). Compared with the control group, the apoptosis rate of breast cancer cells in the polydatin group increased, the levels of apoptotic proteins Bax, c-caspase-3 and caspase-3 increased, and the expression level of miR-181a-5p increased (P<0.05). 【Conclusion】Polydatin inhibits the proliferation and induces apoptosis of breast cancer MDA-MB-231 cells by up regulating miR-181a-5p.

Key words： Breast Neoplasms/PA MicroRNAs POLYGONUM CUSPIDATUM Apoptosis

Received: 12 May 2022

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R737.9

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	LI Fu-cheng
	CHEN Xin-chu
	LI Guan-cheng

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LI Fu-cheng,CHEN Xin-chu,LI Guan-cheng. Study on the Mechanism of Apoptosis of MDA-MB-231 Cells Induced by Polydatin in Vitro Mediated by miR-181a-5p[J]. JOURNAL OF CLINICAL RESEARCH, 2022, 39(7): 1014-1017.

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http://journal07.magtech.org.cn/yxlcyj/EN/10.3969/j.issn.1671-7171.2022.07.015 OR http://journal07.magtech.org.cn/yxlcyj/EN/Y2022/V39/I7/1014

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