糖尿病性白内障晶状体上皮细胞中MMP-9、ET-1的表达水平及其临床意义

doi:10.3969/j.issn.1671-7171.2022.07.016

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Abstract 【Objective】 To investigate the expression and clinical significance of matrix metalloproteinase-9 (MMP-9) and endothelin-1 (ET-1) in diabetic cataract lens epithelial cells. 【Methods】From March 2019 to April 2021, 131 cataract patients accepted in the ophthalmology department of Xianyang First People's Hospital were collected as the research group. According to whether the patients were complicated with diabetes, they were divided into the non-diabetic cataract group of 31 cases and the diabetic cataract group of 100 cases. In addition, 83 cases of ocular trauma requiring removal of the normal transparent lens due to trauma accident were recruited as the control group. The expression of MMP-9 and ET-1 was detected by immunohistochemistry. The correlation of MMP-9 and ET-1 expression in lens epithelial cells of patients with diabetic cataract clinical indicators was analyzed by Spearman method. The related factors affecting diabetic cataract were analyzed by Logistic regression. 【Results】Compared with the control group, the positive expression of MMP-9 and ET-1 in the lens epithelial cells of the non-diabetic cataract group was obviously increased (P<0.05); compared with the non-diabetic cataract group, the positive expression of MMP-9 and ET-1 in the diabetic cataract group was increased (P<0.05). The results of Spearman analysis showed that the expression of MMP-9 and ET-1 was positively associated with fasting blood glucose (FPG), total cholesterol (TC), triglyceride (TG), hemoglobin A1C (HbA1c), high sensitive C-reactive protein (hs-CRP) and malondialdehyde (MDA) (P<0.05), and they were negatively correlated with high-density lipoprotein cholesterol (HDL-C), apolipoprotein A (apoA), superoxide dismutase (SOD) and glutathione (GSH) (P<0.05). Multivariate logistic regression analysis showed that the positive expression of MMP-9 and ET-1 were risk factors for the occurrence of diabetic cataract (P<0.05). 【Conclusion】The expression of MMP-9 and ET-1 in lens epithelial cells of patients with diabetic cataract is increased, which are related to the occurrence of diabetic cataract.

Key words： Diabetes Mellitus/CO Cataract/CO Lens, Crystalline Epithelial Cells Matrix Metalloproteinase 9 Endothelin-1

Received: 08 March 2022

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R776.1

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	CHEN Ning-ning
	ZHAO Ming

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CHEN Ning-ning,ZHAO Ming. Expression and Clinical Significance of MMP-9 and ET-1 in Lens Epithelial Cells of Diabetic Cataract[J]. JOURNAL OF CLINICAL RESEARCH, 2022, 39(7): 1018-1021.

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http://journal07.magtech.org.cn/yxlcyj/EN/10.3969/j.issn.1671-7171.2022.07.016 OR http://journal07.magtech.org.cn/yxlcyj/EN/Y2022/V39/I7/1018

[1] LIM S A,JOO C K,KIM M S,et al. Expression of p53 and caspase-8 in lens epithelial cells of diabetic cataract[J].J Cataract Refract Surg,2014,40(7): 1102-1108.
[2] 陈艳文. 糖尿病性白内障患者血清与房水中细胞因子表达的变化[J].国际眼科杂志,2020,20(11): 1946-1949.
[3] JEONG S R,PARK H Y,KIM Y,et al. Methylglyoxal-derived advanced glycation end products induce matrix metalloproteinases through activation of ERK/JNK/NF-κB pathway in kidney proximal epithelial cells[J].Food Sci Biotechnol,2019,29(5): 675-682.
[4] 周米露,赵锐,王大庆,等. 基于PI3K/AKT信号通路探讨Netrin-1对糖尿病性白内障大鼠晶状体上皮细胞的保护作用[J].解剖学研究,2021,43(6): 577-583.
[5] KWON J W,CHOI J A,JEE D. Matrix metalloproteinase-1 and matrix metalloproteinase-9 in the aqueous humor of diabetic macular edema patients[J].PLoS One,2016,11(7): e0159720.
[6] XUE L P,FU X L,HU M,et al. Rg1 inhibits high glucose-induced mesenchymal activation and fibrosis via regulating miR-2113/RP11-982M15.8/Zeb1 pathway[J].Biochem Biophys Res Commun,2018,501(4): 827-832.
[7] JAMERSON E C,ELHUSSEINY A M,ELSHEIKH R H,et al. Role of matrix metalloproteinase 9 in ocular surface disorders[J].Eye Contact Lens,2020,46(Suppl 2): S57-S63.
[8] LI K,AI W. Investigation of expression and effects of TGF-β1 and MMP-9 in lens epithelial cells of diabetic cataract rats[J].Exp Ther Med,2019,17(6): 4522-4526.
[9] GUGLETA K. Significance of endothelin-1 in glaucoma-a short overview[J].Klin Monbl Augenheilkd,2018,235(2): 140-145.
[10] AHMED M,RGHIGH A. Polymorphism in endothelin-1 gene: an overviewstellenwert von endothelin-1 beim glaukom-ein kurzer überblick significance of endothelin-1 in glaucoma-a short overview[J].Curr Clin Pharmacol,2016,11(3): 191-210.
[11] KHUU L A,TAYYARI F,SIVAK J M,et al. Aqueous humor endothelin-1 and total retinal blood flow in patients with non-proliferative diabetic retinopathy[J].Eye (Lond),2017,31(10): 1443-1450.