Abstract 【Objective】To study the relationship between tumor necrosis factor-α (TNF-α) and lipoprotein lipase (LPL) gene polymorphisms and insulin resistance (IR) in gestational diabetes mellitus (GDM) patients and their newbornsnewborns. 【Methods】54 patients with GDM from October 2018 to October 2019 were included as the observation group, another 54 normal pregnant women were included as the control group. The venous blood of pregnant women were collected, the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) were used to detect the distribution of TNF-α gene -857C/T site, -863A/C site and LPL HindⅢ genotype in pregnant women. The distribution of TNF-αgene -857C/T, -863A/C and LPL HindⅢ were compared between the two groups.The levels of homeostasis model assessment of insulin resistance (HOMA-IR) serum TNF-α and LPL were recorded. The relationship between serum TNF-α, LPL and HOMA-IR in pregnant women were analyzed. 【Results】There were significant differences in genotype and allele distribution of -857C/T locus between the observation group and the control group (P<0.05).The patients with CT genotype and T allele in the observation group were significantly higher than those in the control group, the difference were statistically significant (P<0.05).There were significant difference in the distribution of LPL HindⅢ genotype and allele between the two groups (P<0.05),the patients with H+ H+ genotype and H+ allele in the observation group were significantly higher than those in the control group, the difference were statistically significant (P<0.05).The levels of serum TNF-α, HOMA-IR and newborn HOMA-IR in patients with GDM of CT genotype at -857C/T were significantly higher than those in patients with CC and TT, the serum LPL of patients with H+ H+ genotype of LPL HindⅢ was significantly lower than that of patients with H- H- and H+ genotype, the HOMA-IR and newborn HOMA-IR were significantly higher than H- H- and H+ genotypes, the difference were statistically significant (P<0.05).There were significant positive correlation between serum TNF-α and HOMA-IR of pregnant women and newborns (P<0.05),There were significant negative correlation between LPL and HOMA-IR in pregnant women and newborns (P<0.05). 【Conclusion】The TNF-α gene -857C/T site and LPL Hind Ⅲ gene polymorphisms are related to IR in GDM patients and their newborns. The IR is more likely to occur in patients with TNF-α CT genotype and LPL Hind Ⅲ H+ H+ genotype, which leaded to the occurrence and development of GDM.
ZHANG Jun,ZHANG Li-wen,LI He-ping. Relationship between TNF-α and LPL Gene Polymorphisms and Insulin Resistance in GDM Patients and Their Newborns[J]. JOURNAL OF CLINICAL RESEARCH, 2022, 39(10): 1524-1527.
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2022, Vol. 39 
