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| Expression and Significance of GPR30 in Chorionic Villi of Early Damaged Embryos |
| LIU Li-wen, YANG Chao-lan, LING Yan-zhi |
| Department of Obstetrics and Gynecology,The First Hospital of Changsha City,Changsha,Hunan 410005 |
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Abstract 【Objective】To investigate the expression of G protein-couple receptor (GPR30) in chorionic villi of early pregnancy embryo damage and its correlation with estrogen receptor (ER) and progesterone receptor (PR).【Methods】A total of 100 cases of chorionic villous tissues from March 2017 to March 2018 were selected from the First Hospital of Changsha. There were 50 cases with embryo damage for abortion (the observation group) and 50 cases of normal early pregnancy requiring pregnancy termination (the control group). The expression level of GPR30,ER and PR in villus tissue was detected by immunohistochemical staining and the average optical density value of staining was measured. 【Results】The average optical density values of GPR30,ER and PR staining in the villus tissue of normal early pregnancy group were 0.37±0.07,0.34±0.07 and 0.30±0.05,respectively.The average optical density values of GPR30,ER and PR staining in the embryo damage group were 0.30±0.06, 0.28±0.06 and 0.30±0.04,respectively.The expression levels of GPR30,ER and PR in villus tissue of the normal early pregnancy group were higher than those in the embryo damage group; and the differences were statistically significant (P<0.05). There was a positive correlation between ER and PR expression in the villous tissue of pregnant women with embryo damage for abortion (r=0.486, P=0.000); however, there was no significant correlation of GPR30 with ER and PR expression (r=-0.120,P=0.252; r=-0.027,P=0.794).【Conclusion】The levels of GPR30,ER and PR expression in early pregnancy chorionic villus tissue of abortion caused by embryo damage are significantly lower than those in normal chorionic villi of early pregnancy. There is no significant correlation of GPR30 with ER and PR in the villous tissue of the two groups of pregnant women. The expression of ER shows a positive correlation with PR expression in early pregnancy villus tissue, indicating that the two hormone receptors interact with each other in the process of maintaining pregnancy.
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Received: 28 February 2019
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2020, Vol. 37 
