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| Study on Correlation of Serum Cytokines IL-12 and IL-6 with ET-1 and CEC Levels in Children with Bronchial Asthma Attack |
| CHEN Lu, CHEN Yan-ping, HUANG Jian-bao, et al |
| Department of Respiration, Hunan Children's Hospital, Changsha 410007, Hunan |
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Abstract 【Objective】To investigate the correlation of serum cytokines IL-12 and IL-6 with Endothelin-1( ET-1) and Circulating endothelial cells( CEC)in children with bronchial asthma attack. 【Methods】A total of 90 children with bronchial asthma were divided into the stable group (45 cases) and the attack group (45 cases) according to asthma attack status. The serum levels of IL-12 (the representative cytokine of helper T lymphocyte-1/Th1), IL-6 (the representative cytokine of helper T lymphocyte-1/Th2 ) and ET-1 were measured by ELISA, while the level of CEC was detected by flow cytometry. The levels of Th1/Th2 cytokines (IL-12 and IL-6), ET-1 and CEC were compared between in the two groups. The correlations between serum IL-12 and IL-6 cytokines with levels of ET-1 and CEC were analyzed. 【Results】Level of serum IL-12 in asthma attack group was lower than that in the stable group, while serum IL-6, ET-1 and CEC levels in the attack group were higher than those in the stable stage. The differences between the two groups were statistically significant (P<0.05). The partial correlation coefficient of IL-12 and IL-6 with ET-1 was 0.249, P=0.103, suggesting that IL-12 and IL-6 had a linear trend correlation with ET-1. The partial correlation coefficient of IL-12 and IL-6 with CEC was 0.340, P=0.024, suggesting that IL-12 and IL-6 had a linear correlation with CEC. 【Conclusion】There are significant differences of serum IL-12, IL-6, ET-1 and blood CEC in children with bronchial asthma at different stages (stable vs attack). Serum cytokines IL-12 and IL-6 are closely related to ET-1 and CEC levels in children with bronchial asthma attack stage, which can effectively evaluate the condition of children and guide clinical diagnosis and treatment.
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Received: 21 May 2019
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2019, Vol. 36 
