流式细胞术与不同类型聚合酶链式反应技术用于儿童急性淋巴细胞白血病微小残留病监测的比较

doi:10.3969/j.issn.1671-7171.2021.04.026

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Abstract 【Objective】 To compare the application of flow cytometry (FCM) and polymerase chain reaction (PCR) in monitoring minimal residual disease (MRD) in children with acute lymphoblastic leukemia. 【Methods】 170 patients diagnosed with acute lymphoblastic leukemia in Hunan children's Hospital from January 2016 to December 2018 were collected, and all of them received therapy with SCCLG-ALL-2016 protocol. All patients were detected immunophenotyping by flow cytometry, and detected fusion genes and immunoglobulin (IG) and T cell receptor (TCR) gene rearrangement by PCR at the initial diagnosis. On the 15th day of treatment, MRD was monitored by FCM and different types of PCR (real-time quantitative PCR,RT-qPCR), multiplex PCR and nested RT-PCR). To analyze the differences of MRD monitoring results between FCM and different types of PCR. 【Results】 The positive rate of MRD detected by FCM was higher than that by RT qPCR and nested RT-PCR, but the differences were not statistically significant(P>0.05). The positive rate of MRD detected by FCM was higher than that by multiplex PCR, and the difference was statistically significant (χ²=34.03,P=0.000). OS of MRD positive patients detected by RT-qPCR, nested RT-PCR and FCM was lower than negative patients, but the difference was not statistically significant (P>0.05). OS of MRD positive patients detected by multiplex PCR was lower than negative patients, and the difference was statistically significant (P=0.002). 【Conclusion】 Technique of FCM and RT-qPCR or nested RT-PCR are both useful to monitor MRD level in pediatric ALL,sensitively.The sensitivity of multiplex PCR in MRD is lower than that of FCM.Multiplex PCR detecting IG or TCR gene rearrangement for MRD monitoring has high specificity but low sensitivity.

Key words： Precursor Cell Lymphoblastic Leukemia-Lymphoma/DI Flow Cytometry/MT Multiplex Polymerase Chain Reaction/MT Neoplasm, Residual Child

Received: 09 March 2021

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R733.71

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	SONG Na
	KUANG Wen-yong

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SONG Na,KUANG Wen-yong. Comparision of FCM and Different Types of PCR Techniques in the Monitoring of Small Residual Disease in Children with Acute Lymphocytic Leukemia[J]. JOURNAL OF CLINICAL RESEARCH, 2021, 38(4): 569-571.

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http://journal07.magtech.org.cn/yxlcyj/EN/10.3969/j.issn.1671-7171.2021.04.026 OR http://journal07.magtech.org.cn/yxlcyj/EN/Y2021/V38/I4/569

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