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| Effect of microRNA-132 on Gene Expression Related to Retinal Neovascularization (RNV) in Human Retinal Microvascular Endothelial Cells (HRMECs) under Hypoxia/Reoxygenation (H/R) |
| ZHANG Li-xin |
| Department of Ophthalmology, Hunan Children's Hospital, Changsha, Hunan 410006 |
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Abstract 【Objective】To explore the effect of microRNA-132 on gene expression related to retinal neovascularization (RNV) in human retinal microvascular endothelial cells (HRMECs) under hypoxia/reoxygenation (H/R). 【Methods】HRMECs cell lines were cultured under hypoxia for 6 hours and then under normal oxygen for 6 hours as H/R group; anti-mir-132 was added as H/R + anti-mir-132 group under H/R condition; HRMECs were cultured under normal oxygen condition as negative control group. Total cell RNA was extracted and the changes of gene expression patterns were detected on the RNA microarray. Pathway analysis was performed using Rosetta Resolver 7.2 software package. Finally, candidate genes related to RNV were identified with Pubmed database. 【Results】Compared with the negative control group, there were significant changes in cell signals such as cell adhesion, adipocytokine and extracellular matrix receptor interaction in the H/R group (P<0.05). By inhibiting the expression of microRNA-132, we found that microRNA-132 participated in the regulation of various signal molecules in H/R cells. Three RNV related genes were screened out based on the analysis of Pubmed database: THBS1, IL-6 and CXCL8. 【Conclusion】Mir-132 participates in H/R-induced RNV by regulating the expression of multiple genes, which helps to further elucidate the mechanism of H/R-induced RNV.
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Received: 16 July 2018
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2019, Vol. 36 
