Abstract：【Objective】 To investigate the effect of estrogen on the expression of epithelial mesenchymal transition markers of E-cadherin and vimentin in SPC-A-1 cell line.【Methods】 SPC-A-1 cell line was stimulated with different concentrations of estrogen; the expression of EMT markers was detected by Q-RT-PCR and Western blot; the effect of different concentrations of estrogen on cell migration was detected by Transwell test. The difference between the groups was calculated.【Results】 When the estrogen concentration was 1×10-9 mol/L、1×10-8 mol/L、1×10-7 mol/L, the Vimentin mRNA expression was (2.21±0.53), (4.81±0.66), (2.27±0.51), which was significantly higher than that of the blank control group (P<0.05). The mRNA expression of E-cadherin was (0.67±0.18), (0.42±0.14), (0.61±0.11), which was significantly lower than that of the blank control group (P<0.05). The number of cell migration was (23.65±3.25), (37.88±5.66) and (22.17±2.98) respectively (P<0.05). When the estrogen concentration was 1×10-8 mol/L, the expression of vimentin was the highest, the expression of E-cadherin was the lowest, and the number of cell migration was the highest.【Conclusion】 Appropriate concentration of estrogen can promote the expression of vimentin, inhibit the expression of E-cadherin, and improve the ability of cell migration. When the concentration of estrogen is 1×10-8 mol/L, the effect of promoting vimentin expression, inhibiting E-cadherin expression and promoting cell migration is most significant. Estrogen can promote the EMT process of SPC-A-1 cell line.
张继朋, 卢强, 陈鹏, 李小飞, 陈静, 王武平, 杨三虎. 雌激素对肺腺癌SPC-A-1细胞上皮间质转化标志物Vimentin、E-cadherin表达量的影响[J]. 医学临床研究, 2020, 37(10): 1450-1453.
ZHANG Ji-peng, LU Qiang, CHEN Peng, et al. Effect of Estrogen on the ENT Expression of Vimentin and E-cadherin in Lung Adenocarcinoma SPC-A-1 cells. JOURNAL OF CLINICAL RESEARCH, 2020, 37(10): 1450-1453.
[1] Farbod EA, Saber AA, Fatemeh PA， et al.Cost-effectiveness oflung cancerscreening and treatment methods: a systematicreviewof systematic reviews[J].BMC Health Serv Res,2017, 17(1): 413. [2] Anna MR, Anna D. Multiple primarylung cancer: A literaturereview[J].Adv Clin Exp Med,2018, 27(5): 725-730. [3] Turley EA, Veiseh M, Radisky DC, et al. Mechanisms of disease: epithelial-mesenchymal transition-does cellular plasticity fuel neoplastic progression[J].Nat Clin Pract Oncol,2008, 5(5): 280-290. [4] 魏小义, 姚家喜,秦玉洁,等.Vimentin在转移性肾癌中的表达及预后意义[J].国际外科学杂志,2020,47(9):589-591. [5] Honma N, Hosoi T, Arai T, et al. Estrogen and cancers of the colorectum, breast, and lung in postmenopausal women[J].Pathol Int,2015, 65(6): 451-459. [6] Siegel RL, Miller KD,Jemal A. Cancer statistics, 2016[J].Ca Cancer J Clin,2016, 66(6): 7-30. [7] Chakraborty S, Ganti AK, Marr A, et al. Lung cancer in women: role of estrogens[J].Expert Rev Respir Med,2010, 4(4): 509-518. [8] Reinmuth N, Heigener DF,Reck M. Non small cell lung cancer news from immunotherapy[J].Dtsch Med Wochenschr,2015, 140(33): 329-333. [9] Rodriguez AZ, Maurer KA, Gertz J. Estrogen signaling in endometrial cancer: a key oncogenic pathway with several open questions[J].Horm Cancer,2019, 10(2): 51-63. [10] Eylem KC,Alexandra A,Zeynep ME. Crosstalk between estrogen signaling and breast cancer metabolism[J].Trends Endocrinol Metab,2019, 30(1): 25-38. [11] Greenburg G,Hay ED. Epithelia suspended in collagen gels can lose polarity and express characteristics of migrating mesenchymal cells[J].J Cell Biol,1982, 95(23): 333-339. [12] Li L,Li W. Epithelial-mesenchymal transition in human cancer comprehensive reprogramming of metabolism, epigenetic and differentiation[J].Pharmacol Therapeut,2015, 150: 33-46. [13] Kay TY, Jing Y. Epithelial mesenchymal transitionin tumor metastasis[J].Annu Rev Pathol,2018, 24(5): 395-412. [14] Yang YM , Yang WX . Epithelial-to-mesenchymaltransitionin the development of endometriosis[J].Oncotarget,2017, 8(25): 41679-41689. [15] Heather CB.Loss of E-cadherin-dependent cell-cell adhesion and the development and progression of cancer[J].Cold Spring Harb Perspect Biol,2018, 10(3): 553-559. [16] Yin S, Chen FF, Yang GF. Vimentinimmunohistochemical expression as a prognostic factor in gastriccancer: A meta-analysis[J].Pathol Res Pract,2018, 214(9): 1376-1380.
2020, Vol. 37 
