Expression of IL-22 of the from Peripheral Blood Mononuclear Cells in Patients with Rheumatic Heart Disease
DUAN Chang-en, SU Guo-bao, ZHOU Chao-yuan,et al
Department of Cardiovascular Surgery, the First Affiliated Hospital of Xinxiang Medical University & Henan Key Laboratory of Neurol Regeneration, Weihui 453100, China
Abstract：【Objective】To investigate the expression and significance of the interleukin-22 that secreted by the peripheral blood mononuclear cells from the patients with rheumatic heart disease (RHD) to provide theoretical basis for the clinical treatment. 【Methods】A total of 30 patients with RHD who underwent surgery from May 2016 to May 2017 were selected as the observation group and 30 healthy blood donors were selected as the control group. Peripheral blood mononuclear cells from patients with rheumatic heart disease and healthy donors were isolated by density gradient centrifugation. The content and gene expression of IL-22 in the culture medium were detected using ELISA and qRT-PCR, respectively. At the same time, the cellular morphology of peripheral blood mononuclear were observed by scanning electron microscope after co-cultured with the culture medium from patients with rheumatic heart disease . 【Results】The content and gene expression of IL-22 in the culture medium of patients with rheumatic heart disease were significantly higher than those in healthy donors. The differences were statistically significant(P＜0.05). The morphology of peripheral blood mononuclear cells from healthy donors showed dramatically changed even collapsed and ruptured when they co-cultured with the secreted medium from RHD patients' mononuclear cells.. 【Conclusion】The over-expression of IL-22 of the peripheral blood mononuclear cells from patients with rheumatic heart disease plays a certain role in the occurrence and development of the disease RHD.
段长恩, 苏国宝, 周朝元, 韩培立. 风湿性心脏病患者外周血单个核细胞IL-22的表达及其意义[J]. 医学临床研究, 2019, 36(8): 1457-1459.
DUAN Chang-en, SU Guo-bao, ZHOU Chao-yuan,et al. Expression of IL-22 of the from Peripheral Blood Mononuclear Cells in Patients with Rheumatic Heart Disease. JOURNAL OF CLINICAL RESEARCH, 2019, 36(8): 1457-1459.
[1] Dumoutier L, Lejeune D, Colau D,et al. Cloning and characterization of IL-22 binding protein, a natural antagonist of IL-10-related T cell-derived inducible factor/IL-22[J].J Immunol,2001,166(12):7090-7095. [2] Chichelnitskiy E, Himmelseher B,Bachmann M,et al. Hypothermia Promotes Interleukin-22 Expression and Fine-Tunes Its Biological Activity[J].Front Immunol,2017,8:742. [3] Batzloff MR, Fane A, Gorton D,et al. Preclinical immunogenicity and safety of a Group A streptococcal M protein-based vaccine candidate [J].Hum Vaccin Immunother,2017,19(5):287-290. [4] 王文,朱曼璐,王拥军,等.中国心血管病报告2012概要[J].中国循环杂志,2013,28,6:408-412. [5] 信国玺,张宗泰,苗洪志,等. 风湿性心脏病二尖瓣置换术若干问题探讨[J].齐齐哈尔医学院学报,2002,23(6):687. [6] Witte E, Witte K,Warszawska K,et al. Interleukin-22: a cytokine produced by T, NK and NKT cell subsets, with importance in the innate immune defense and tissue protection[J].Cytokine Growth Factor Rev,2010,21(5):365-379. [7] Kong Q, Li X, Wu W, et al. Increased circulating T helper 22 cells in patients with dilated cardiomyopathy[J].Mol Med Rep,2014,10(1):359-364. [8] 陈志忠,李结敏,陈尚良,等.病毒灭活血浆残余亚甲蓝对人外周血单个核细胞免疫功能影响的体外研究[J].国际检验医学杂志,2016,37(16):2205-2207. [9] 马星星,薛云,张义和.风湿性心脏病二尖瓣置换手术前后患者treg及il-6、il-10、TGF-β1的变化分析[J].检验医学与临床,2016,13(18):2613-2615. [10] 彭桉平,卢欣沂,何敏,等.IL-22诱导类风湿关节炎患者滑膜成纤维细胞分泌IL-6促进Th17细胞分化的研究[J].中华微生物学和免疫学杂志,2014,34(7):541-545.
2019, Vol. 36 
