Abstract：【Objective】To investigate the expression of G protein-couple receptor (GPR30) in chorionic villi of early pregnancy embryo damage and its correlation with estrogen receptor (ER) and progesterone receptor (PR).【Methods】A total of 100 cases of chorionic villous tissues from March 2017 to March 2018 were selected from the First Hospital of Changsha. There were 50 cases with embryo damage for abortion (the observation group) and 50 cases of normal early pregnancy requiring pregnancy termination (the control group). The expression level of GPR30,ER and PR in villus tissue was detected by immunohistochemical staining and the average optical density value of staining was measured. 【Results】The average optical density values of GPR30,ER and PR staining in the villus tissue of normal early pregnancy group were 0.37±0.07,0.34±0.07 and 0.30±0.05,respectively.The average optical density values of GPR30,ER and PR staining in the embryo damage group were 0.30±0.06, 0.28±0.06 and 0.30±0.04,respectively.The expression levels of GPR30,ER and PR in villus tissue of the normal early pregnancy group were higher than those in the embryo damage group; and the differences were statistically significant (P<0.05). There was a positive correlation between ER and PR expression in the villous tissue of pregnant women with embryo damage for abortion (r=0.486, P=0.000); however, there was no significant correlation of GPR30 with ER and PR expression (r=-0.120,P=0.252; r=-0.027,P=0.794).【Conclusion】The levels of GPR30,ER and PR expression in early pregnancy chorionic villus tissue of abortion caused by embryo damage are significantly lower than those in normal chorionic villi of early pregnancy. There is no significant correlation of GPR30 with ER and PR in the villous tissue of the two groups of pregnant women. The expression of ER shows a positive correlation with PR expression in early pregnancy villus tissue, indicating that the two hormone receptors interact with each other in the process of maintaining pregnancy.
刘丽文, 杨超兰, 凌艳芝. GPR30在胚胎停育早孕绒毛组织中的表达及其意义[J]. 医学临床研究, 2020, 37(1): 39-41.
LIU Li-wen, YANG Chao-lan, LING Yan-zhi. Expression and Significance of GPR30 in Chorionic Villi of Early Damaged Embryos. JOURNAL OF CLINICAL RESEARCH, 2020, 37(1): 39-41.
[1] 刘丽娜,张丽颖.胚胎停育高危因素和并发症的探索[J].疑难病杂志 ,2015,14(2):214-217. [2] Plante BJ,Lessey BA,Taylor RN,et al. G protein-coupled estrogen receptor (GPER) expression in normal and abnormal endometrium[ J].Reprod Sci,2012,19(7) :684-693. [3] 谢幸,苟文丽.妇产科学[M].第8版.北京:人民卫生出版社,2013. [4] 常玉婷,巧信勇,孙明英.胎停育的早期诊断与处理[J].临床医学,2012,22(10):40. [5] Pridjian G.Missed abortion:still appropriate terminology[J]?Am J Obsetet Gyned,1989,16(2):261-262. [6] 付昱,黄明莉,袁晶.早孕期胚胎停育主要病因及治疗的研究进展[J].中华妇幼临床医学杂志,2015,11(2):110-113. [7] Wang D,Hu L,Zhang G,et al.G protein-coupled receptor 30 intumor development[J].Endocrine,2010,38(1):29-37. [8] Prosnitz ER,Maggiolini M.Mechanisms of estrogen signaling via GPR30[J].Mol Cell Endocrinol,2009,308(1/2):32-38. [9] Lappano R,De Marco P,De Francesco EM,et al.Cross-talk between GPER and growth factor signaling [J].J Steroid Biochem Mol Biol,2013,137:50-56. [10] Frick KM,Kim J,Tuschuer JJ,et al.Sex steroid hormones matter for learning and memory:estrogenic regulation of hippocampal function in male and female rodents regulation of hippocampal function in male and female rodents [J].Learn Mem,2015,22(9):472-493. [11] Qu T,Zhang SM,Yu LL,et al.Relocalisation and activation of integrins induced rapidly by ocestrogen via G-protein-coupled receptor 30 in mouse blastocystg[J].Reprod Fertil Dev,2015,28(11):1679-1685. [12] Tropea T,De Francesco EM,et al.Pregnancy augments g protein estrogen receptor (GPER) induced vasodilation in rat uterine arteries via the nitric oxide-cGMP signaling pathway[J].PLoS One,2015,10(11):e0141997. [13] 李飞,罗浩军,罗平,等.雌激素受体GPR30、ERα和PR在甲状腺癌组织中的表达及相关性[J].第三军医大学学报,2011,33(16):1691-1694.
2020, Vol. 37 
