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| Expression Level and Significance of circ_0000847 in Lung Cancer Tissues |
| DING Lingyan, TANG Zhaohui, LI Lizhi |
| Department of Oncology, Yongzhou Central Hospital, Yongzhou Hunan 425000 |
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Abstract 【Objective】 To investigate the expression level and significance of circ_0000847 in lung cancer tissues. 【Methods】 Lung cancer tissues and adjacent normal tissues were collected from 29 patients with lung cancer who underwent surgical treatment in our hospital from June 2017 to June 2022. Normal bronchial epithelial cell line HBE and lung cancer cell lines H1299 and A549 were selected. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression level of circ_0000847 in each tissue and cell line. H1299 and A549 cells in logarithmic growth phase were transfected with si-NC (si-NC group) and si-circ_0000847 (si-circ_0000847 group), respectively. At 48 hours after transfection, methyl thiazolyl tetrazolium (MTT) colorimetry, flow cytometry and Transwell assay were used to detect and compare cell viability, apoptosis and invasion ability. 【Results】 The relative expression level of circ_0000847 in lung cancer tissues was significantly higher than that in adjacent tissues (P<0.05). Before transfection, the relative expression levels of circ_0000847 in H1299 and A549 cells were significantly higher than those in HBE cells (P<0.05). After transfection, compared with the si-NC group, the relative expression level of circ_0000847 in H1299 and A549 cells in the si-circ_0000847 group was significantly decreased (P<0.05). Compared with the si-NC group, the cell viability of H1299 and A549 cells in the si-circ_0000847 group was decreased (P<0.05), the apoptosis rate was increased (P<0.05), and the number of invasive cells was decreased (P<0.05). 【Conclusion】 circ_0000847 is highly expressed in lung cancer tissues and is involved in the proliferation and invasion of lung cancer cells. Interference with circ_0000847 expression reduces the proliferation and invasion of lung cancer cells and increases their apoptosis.
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Received: 07 November 2024
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. [J]. JOURNAL OF CLINICAL RESEARCH, 2025, 42(9): 1667-1669. |
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2025, Vol. 42 
