Abstract 【Objective】To investigate the impact of SEPT9 gene methylation level in cancer tissues on progression-free survival after surgery in patients with stage Ⅱ-Ⅲ resectable rectal cancer. 【Methods】A total of 129 patients who underwent R0 total mesorectal excision for stage Ⅱ-Ⅲ resectable rectal cancer were followed up for 5 years postoperatively. The positive expression of SEPT9 gene methylation in cancer tissues and adjacent non-cancerous tissues was compared, and the relationship of SEPT9 gene methylation levels in cancer tissues with clinicopathological parameters was analyzed. Factors affecting postoperative recurrence or metastasis of rectal cancer were investigated, along with the relationship between positive expression of SEPT9 gene methylation in cancer tissues and progression-free survival after surgery. 【Results】The positive expression rate of SEPT9 gene methylation in cancer tissues was higher than that in adjacent non-cancerous tissues(P<0.05). Patients with poorly differentiated, N2 stage, stage Ⅲ, and tumor invasion into the vasculature had higher positive expression rates of SEPT9 gene methylation in cancer tissues than those with moderately to well-differentiated, N0-N1 stage, stage Ⅱ, and no tumor invasion into the vasculature(P<0.05). Cox regression analysis showed that preoperative carcinoembryonic antigen levels, pathological staging, differentiation degree, and positive SEPT9 gene methylation were independent factors affecting postoperative recurrence or metastasis(P<0.05). Patients with positive expression of SEPT9 gene methylation had lower progression-free survival rates than those with negative expression(P<0.05). 【Conclusion】The level of SEPT9 gene methylation in cancer tissues of patients with stage Ⅱ-Ⅲ resectable rectal cancer is related to clinicopathological parameters and prognosis. Patients with positive expression of SEPT9 gene methylation have a higher risk of postoperative recurrence and metastasis.
LI Yueping,HAN Bo,ZHANG Rumeng, et al. Effect of SEPT9 Gene Methylation Level in Cancer Tissue on postoperative Progression-free Survival in Patients with Stage Ⅱ-Ⅲ Resectable Rectal Cancer[J]. JOURNAL OF CLINICAL RESEARCH, 2024, 41(3): 375-378.
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2024, Vol. 41 
