癌组织中SEPT9基因甲基化水平对Ⅱ~Ⅲ期可切除直肠癌术后无进展生存期的影响

doi:10.3969/j.issn.1671-7171.2024.03.015

Abstract
Figure/Table
References (0)
Related Citation (15)

Download: PDF (1158 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract 【Objective】To investigate the impact of SEPT9 gene methylation level in cancer tissues on progression-free survival after surgery in patients with stage Ⅱ-Ⅲ resectable rectal cancer. 【Methods】A total of 129 patients who underwent R0 total mesorectal excision for stage Ⅱ-Ⅲ resectable rectal cancer were followed up for 5 years postoperatively. The positive expression of SEPT9 gene methylation in cancer tissues and adjacent non-cancerous tissues was compared, and the relationship of SEPT9 gene methylation levels in cancer tissues with clinicopathological parameters was analyzed. Factors affecting postoperative recurrence or metastasis of rectal cancer were investigated, along with the relationship between positive expression of SEPT9 gene methylation in cancer tissues and progression-free survival after surgery. 【Results】The positive expression rate of SEPT9 gene methylation in cancer tissues was higher than that in adjacent non-cancerous tissues(P<0.05). Patients with poorly differentiated, N₂ stage, stage Ⅲ, and tumor invasion into the vasculature had higher positive expression rates of SEPT9 gene methylation in cancer tissues than those with moderately to well-differentiated, N₀-N₁ stage, stage Ⅱ, and no tumor invasion into the vasculature(P<0.05). Cox regression analysis showed that preoperative carcinoembryonic antigen levels, pathological staging, differentiation degree, and positive SEPT9 gene methylation were independent factors affecting postoperative recurrence or metastasis(P<0.05). Patients with positive expression of SEPT9 gene methylation had lower progression-free survival rates than those with negative expression(P<0.05). 【Conclusion】The level of SEPT9 gene methylation in cancer tissues of patients with stage Ⅱ-Ⅲ resectable rectal cancer is related to clinicopathological parameters and prognosis. Patients with positive expression of SEPT9 gene methylation have a higher risk of postoperative recurrence and metastasis.

Key words： Rectal Neoplasms/SU DNA Methylation Survival Rate

Received: 25 October 2023

PACS:

R735.37

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LI Yueping
	HAN Bo
	ZHANG Rumeng
	et al

Cite this article:

LI Yueping,HAN Bo,ZHANG Rumeng, et al. Effect of SEPT9 Gene Methylation Level in Cancer Tissue on postoperative Progression-free Survival in Patients with Stage Ⅱ-Ⅲ Resectable Rectal Cancer[J]. JOURNAL OF CLINICAL RESEARCH, 2024, 41(3): 375-378.

URL:

http://journal07.magtech.org.cn/yxlcyj/EN/10.3969/j.issn.1671-7171.2024.03.015 OR http://journal07.magtech.org.cn/yxlcyj/EN/Y2024/V41/I3/375

[1] NIIDA A, MIMORI K, SHIBATA T, et al. Modeling colorectal cancer evolution[J].J Hum Genet,2021, 66(9):869-878.
[2] REYNGOLD M, NILAND J, TER VEER A, et al. Trends in intensity modulated radiation therapy use for locally advanced rectal cancer at National Comprehensive Cancer Network centers[J].Adv Radiat Oncol,2017, 3(1):34-41.
[3] PETERSE E F P, MEESTER R G S, DE JONGE L, et al. Comparing the cost-effectiveness of innovative colorectal cancer screening tests[J].J Natl Cancer Inst,2021, 113(2):154-161.
[4] SOBHANI I, BERGSTEN E, COUFFIN S, et al Colorectal cancer-associated microbiota contributes to oncogenic epigenetic signatures[J].Proc Natl Acad Sci U S A,2019116(48):24285-24295.
[5] BACH S, PAULIS I, SLUITER N R,et al. Detection of colorectal cancer in urine using DNA methylation analysis[J].Sci Rep,2021, 11(1):2363-2369.
[6] 谭琪, 宗明, 虞珊珊, 等. 联合检测外周血游离Septin9、SDC2、BCAT1基因甲基化在结直肠癌诊断中的意义[J].中华检验医学杂志, 2021, 44(3): 204-211.
[7] 张桂前, 孙鷖, 朱红艳, 等. 血浆SEPT9基因甲基化检测在结直肠癌筛查中的应用分析[J].中华肿瘤防治杂志, 2020, 27(15):1263-1268.
[8] 中华医学会消化内镜学分会.中国早期结直肠癌筛查及内镜诊治指南(2014,北京)[J].中华医学杂志, 2015, 95(28):2235.
[9] 贺娜,冯巩,窦建华,等. DNMT3b表达与SEPT9基因甲基化在结直肠癌发生进程中的相关性[J].中华肿瘤杂志,2020,42(11):925-930.
[10] 美国结直肠癌多中心工作组和美国癌症协会, 帅晓玮, 谢鹏雁. 结肠癌切除术后结肠镜随访方案指南:美国结直肠癌多中心工作组和美国癌症协会的最新共识(2006年)[J].中西医结合结直肠病学, 2008, 2(2): 378-379.
[11] KOTOH Y, SUEHIRO Y, SAEKI I, et al. Novel liquid biopsy test based on a sensitive methylated SEPT9 assay for diagnosing hepatocellular carcinoma[J].Hepatol Commun,2020, 4(3):461.
[12] 吴秀方, 南琼, 张晓红, 等. 血浆SEPT9甲基化检测对结直肠癌诊断价值的临床研究[J].中国全科医学, 2021, 24(15):1915-1919.
[13] SHAFIEI M, ALEMRAJABI M, NAJAFI A, et al. Candidate molecular biomarkers for the non-invasive detection of colorectal cancer using gene expression profiling[J].Iran J Pathol,2021, 16(2):205-214.
[14] SUN J, ZHENG M Y, LI Y W, et al. Structure and function of Septin 9 and its role in human malignant tumors[J].World J Gas Oncol,2020, 12(6):619-631.
[15] YANG X, XU Z J, CHEN X, et al. Clinical value of preoperative methylated septin 9 in Chinese colorectal cancer patients[J].World J Gas,2019, 25(17):2099-2109.