PAR-1、PAR-2在先天性巨结肠组织中的表达及其与预后的相关性分析

doi:10.3969/j.issn.1671-7171.2023.01.019

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (1136 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract 【Objective】 To investigate the expression of protease activated receptor-1 (PAR-1) and protease activated receptor-2 (PAR-2) in Hirschsprung's disease (HSCR) and their relationship with prognosis. 【Methods】 A total of 100 children with HSCR who received radical surgery in our hospital from January 2014 to January 2019 were selected, and tissue samples of HSCR lesions (spastic segments) and normal intestinal mucosa adjacent to the lesions (more than 2 cm from the lesions) were taken. Immunohistochemistry was used to detect the expression of PAR-1 and PAR-2 in HSCR pathological tissue samples and normal intestinal mucosa tissues. Fluorescent quantitative PCR was used to detect the expression of PAR-1 mRNA and PAR-2 mRNA. Pearson's method was used to analyze the horizontal correlation between PAR-1, PAR-2, Krickenbeck scores, serum IL-6 and TNF-α. Logistic multivariate analysis was used to analyze the risk factors of poor prognosis in children with HSCR. 【Results】 The positive expression rates of PAR-1 and PAR-2 in the pathological tissues were higher than those in the adjacent tissues (P<0.05). Compared with the adjacent tissues, the expression levels of PAR-1 mRNA, PAR-2 mRNA and PAR-1, PAR-2 protein in the diseased tissues were significantly higher (P<0.05); The level of PAR-1, PAR-2 and serum IL-6, TNF-α in children in the poor prognosis group was significantly higher than that of the good prognosis group and the better prognosis group (P<0.05). The good prognosis group was higher than the better prognosis group (P<0.05). Pearson's results showed that PAR-1, PAR-2 were negatively correlated with Krickenbeck's score (P<0.05), and were positively correlated with IL-6 and TNF-α (mean P<0.05). Multivariate logistic regression analysis showed that PAR-1 and PAR-2 were independent risk factors for poor prognosis of children with HSCR (P<0.05). 【Conclusion】 PAR-1 and PAR-2 are highly expressed in HSCR tissues and negatively correlated with Krickenbeck score, which are risk factors for poor prognosis of children with HSCR.

Key words： Hirschsprung Disease/PA Receptor, PAR-1 Receptor, PAR-2 Prognosis

Received: 14 December 2021

PACS:

R726.2

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	JI Yun-feng
	HUI Sheng-qi

Cite this article:

JI Yun-feng,HUI Sheng-qi. Expression of PAR-1 and PAR-2 in Hirschsprung's Disease and its Correlation with Prognosis[J]. JOURNAL OF CLINICAL RESEARCH, 2023, 40(1): 64-67.

URL:

http://journal07.magtech.org.cn/yxlcyj/EN/10.3969/j.issn.1671-7171.2023.01.019 OR http://journal07.magtech.org.cn/yxlcyj/EN/Y2023/V40/I1/64

[1] 张彦,夏慧敏.先天性巨结肠的遗传发病机制及前瞻性队列研究进展[J].临床小儿外科杂志,2018,17(2):90-93.
[2] PARAHITA I G,MAKHMUDI A,GUNADI,et al.Comparison of hirschsprung-associated enterocolitis following soave and duhamel procedures[J].J Pediatr Surg,2018,53(7):1351-1354.
[3] PRATO A P,BARTOW-MCKENNEY C,HUDSPETH K,et al.A metagenomics study on hirschsprung's disease associated enterocolitis:biodiversity and gut microbial homeostasis depend on resection length and patient's clinical history[J].Front Pediatr,2019,7(1):326-335.
[4] KLINNGAM W,FU R,JANGA S,et al.Cathepsin S alters the expression of pro-inflammatory cytokines and MMP-9,partially through protease-activated receptor-2,in human corneal epithelial cells[J].Int J Mol Sci,2018,19(11):3530-3554.
[5] 李建军.不同类型胃食管反流患者食管组织PAR-2和COX-2表达及与炎性因子的相关性[J].现代中西医结合杂志,2020,29(21):2320-2324.
[6] ADAMS G N,KRISHAN S B,LEAH R,et al.Protease activated receptor-1 impedes prostate and intestinal tumor progression in mice[J].J Thromb Haemost,2018,16(1):2258-2269.
[7] XIAO T,ZHANG Q,ZONG S M,et al.Protease-activated receptor-1 (PAR1) promotes epithelial-endothelial transition through Twist1 in hepatocellular carcinoma[J].J Exp Clin Cancer Res,2018,37(1):185-196.
[8] 王凤青,张波,张凡庆,等.PAR-2在哺乳动物肠道炎症中的作用机制研究进展[J].动物医学进展,2018,39(10):91-94.
[9] ADAMS M N,RAMACHANDRAN R,YAU M K,et al.Structure,function and pathophysiology of protease activated receptors[J].Pharmacol Ther,2011,130(3):248-282.
[10] CHRISTIAN S,MICHAEL S,GEORG G,et al.Inhibition of protease-activated receptor (PAR1) reduces activation of the endothelium,coagulation,fibrinolysis and inflammation during human endotoxemia[J].Thromb Haemost,2018,118(7):1176-1184.
[11] 胡星,王昕,邬四明.血清TNF-α、IL-6和IL-8炎症因子表达对先天性巨结肠相关性小肠结肠炎的临床意义[J].中国现代普通外科进展,2017,20(3):211-212.
[12] 严然.血清IL-6和IL-8炎症因子表达对先天性巨结肠相关性小肠结肠炎的临床价值分析[J].数理医药学杂志,2019,32(2):206-207.