胸腺肽α1对铜绿假单胞菌诱导的支气管扩张大鼠免疫失衡的干预作用

doi:10.3969/j.issn.1671-7171.2022.04.016

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Abstract 【Objective】To explore interventional effect of thymosin α1 and participation of high mobility group box B1 (HMGB1)/receptor advanced glycation end products (RAGE)/Toll-like receptor 9 (TLR9) signaling pathway on immune imbalance in rats with pseudomonas aeruginosa-induced bronchiectasis. 【Methods】A total of 60 healthy male rats were divided into the blank control group, model group, low-dose and high-dose thymosin groups, with 15 cases in each group. Except blank control group, all rats in the other groups were given tracheal perfusion of Pseudomonas aeruginosa to induce bronchiectasis models. The low-dose and high-dose thymosin groups were intraperitoneally injected with 1.25 and 2.50 mg/kg thymosin α1, while blank control group and the model group were injected with the same volume of normal saline for 20d. At 24h after the last administration, changes of respiratory function monitoring indexes were detected. The levels of serum HMGB1, RAG and interferon-γ (IFN-γ) were detected by ELASA. The distribution of T cell subsets was detected by flow cytometry. The expressions of HMGB1 and RAGE in bronchial tissues were detected by Western blot. 【Results】Compared with the blank control group, the tidal volume, minute ventilation volume, IFN-γ, CD3⁺, CD4⁺ and CD4⁺/CD8⁺ levels decreased in model group (P<0.05), while respiratory rate, levels of HMGB1 and RAGE, expressions of HMGB1 and TLR9 increased (P<0.05).However, there was no significant change in CD8⁺ percentage (P>0.05). Compared with the model group, tidal volume, minute ventilation volume, IFN-γ, CD3⁺, CD4⁺ and CD4⁺/CD8⁺ levels increased in low-dose and high-dose thymosin group (P<0.05), while respiratory rate, levels of HMGB1 and RAGE and CD8⁺, expressions of HMGB1 and TLR9 decreased (P<0.05). 【Conclusion】Intraperitoneal injection of thymosin α1 can prevent the cascade effect of cytokines and inflammatory mediators in bronchiectasis rats induced by Pseudomonas aeruginosa through inhibiting HMGB1/RAGE/TLR9 signaling pathway, which can improve immune function in rats.

Key words： Bronchiectasis Pseudomonas aeruginosa/IM Disease Models, Animal Immunoassay

Received: 14 December 2021

PACS:

R562.22

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	LYU Jian-ning
	LI Qiong

Cite this article:

LYU Jian-ning,LI Qiong. Effect of Thymosin α1 on Immune Imbalance in Rats with Pseudomonas Aeruginosa-induced Bronchiectasis[J]. JOURNAL OF CLINICAL RESEARCH, 2022, 39(4): 535-538.

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http://journal07.magtech.org.cn/yxlcyj/EN/10.3969/j.issn.1671-7171.2022.04.016 OR http://journal07.magtech.org.cn/yxlcyj/EN/Y2022/V39/I4/535

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