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| Effect of Sodium Selenite on the Morphology of the Substantia Nigra and the Content of Glutamate in the Striatum of Rats with Parkinson's Disease |
| FAN Kai |
| Northern Hospital of Xi'an City, Shaanxi Province, Xi'an 710032 |
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Abstract 【Objective】To observe the effect of sodium selenite on the morphology of the substantia nigra and the content of glutamate in the striatum of Parkinson's disease (PD) rats, and to explore the related mechanisms. 【Methods】A PD rat model was established by injecting 6-Hydroxydopamine (6-OHDA). Twenty successfully modeled rats were randomly divided into the model group and the experimental group, with 10 rats in each group. Ten rats were injected with saline only and set as the control group. Fifteen days after the injection, the experimental group were given sodium selenite 100 mg/kg, while the control group and model group were given an equal volume of normal saline, once a day for 30 consecutive days. The cognitive function was detected by T-maze experiment. The content of glutamate in the striatum were detected by high performance liquid chromatography (HPLC). The relative expression of substantia nigra c-Jun N-terminal kinase (JNK) and p-JNK protein were detected by Western blotting. 【Results】The accuracy rates of the T-maze experiment in the control group, the model group and the experimental group were (94.44±5.27)%, (50.00±6.39)% and (72.22±8.42)%, respectively. Of which, the content of glutamate in the striatum were (15.96±2.79)μg/mL, (31.87±4.21)μg/mL and (23.34±3.92)μg/mL. The substantia nigra p-JNK/JNK in the control group, the model group and the experimental group were 0.19±0.02,0.78±0.08 and 0.27±0.03, respectively. The differences of the correct rate of T-maze experiment, striatal glutamate content, substantia nigra p-JNK/JNK ratio between the model group and the control group were statistically significant (P<0.05). They were also significant differences between the model group and the experimental group(P<0.05). 【Conclusion】Sodium selenite can improve the cognitive function of PD rats in terms of the morphology of substantia nigra neurons, protect the function of substantia nigra dopaminergic neurons, and reduce the content of striatal glutamate, which may be achieved by inhibiting the JNK signaling pathway.
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Received: 15 November 2021
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2022, Vol. 39 
