Abstract【Objective】 To explore the mechanism of CD8+ T lymphocyte apoptosis in peripheral blood of patients with primary liver cancer. 【Methods】 Sixty four patients with primary liver cancer who were treated in our hospital from July 2016 to July 2018 were selected as the observation group, and 62 healthy volunteers who received physical examination in our hospital during the same period were selected as the control group. The proportion of peripheral blood CD8+ T lymphocyte and the apoptosis ratio of peripheral blood CD8+ T lymphocyte were compared.【Results】 The proportion of peripheral blood CD8+ T lymphocytes in the observation group was lower than that in the control group, and the apoptosis rate of peripheral blood CD8+ T lymphocytes in the observation group was significantly higher than that in the control group (P<0.05). The proportion of CD8+ T lymphocytes in peripheral blood of TNM stageⅠ-Ⅱ group was significantly higher than that of stageⅢ-Ⅳ group, and the apoptosis rate of peripheral blood CD8+ T lymphocyte was significantly lower than that of stage Ⅲ-Ⅳ (P<0.05). The proportion of peripheral blood CD8+ T lymphocytes in the observation group was lower than that in the control group, and the apoptosis rate of peripheral blood CD8+T cells in the observation group was higher than that in the control group (P<0.05).【Conclusion】 The proportion of CD8+ T lymphocytes in peripheral blood of patients with primary liver cancer is lower than that of healthy people, and the apoptosis of CD8+ T lymphocytes in patients with TNM stage Ⅲ-Ⅳ is more serious.
YU Kuai,LI Qiu. Analysis of the Proportion and Apoptosis of Peripheral Blood CD8+ T Lymphocytes in Patients with Primary Liver Cancer[J]. JOURNAL OF CLINICAL RESEARCH, 2020, 37(9): 1330-1332.
[1] 陈宏伟,杨孙虎.异甘草酸镁联合丁二磺酸腺苷蛋氨酸对原发性肝癌介入术后肝功能及预后的影响[J].医学临床研究,2020,37(2):199-202. [2] 方大正,吴红伟,武伦,等.肝癌患者CD8+CD28-Foxp3+调节性T细胞的变化及意义[J].中国普通外科杂志,2017,26(7):870-876. [3] 张赫,邹寒冰,刘喆,等.50例肝癌患者外周血淋巴细胞亚群比例与肝功能损伤的关系[J].肝脏,2017,22(11):994-997. [4] Sanaz N, Hadi B. Natural killer or T-lymphocyte cells: which is the best immune therapeutic agent for cancer? An optimal control approach[J].Int J Control Autom Syst,2014,12(1):84-92. [5] 陈燕,胡浩,张诗军,等.四君子汤对原发性肝癌术后患者T淋巴细胞和NK细胞的影响及意义[J].中西医结合肝病杂志,2017,27(1):8-10. [6] 郭存丽,毕玉美,刘钊,等.肝细胞肝癌患者外周血CD8+ T淋巴细胞凋亡相关机制探讨[J].现代肿瘤医学,2018,26(14):2151-2155. [7] Gadhamsetty S, Marée AFM.,Beltman JB,et al. A general functional response of cytotoxic T lymphocyte-mediated killing of target cells[J].Biophys J,2014,106(8):1780-1791. [8] 李淑群,翁俊,莫庆荣,等.GP73在肝癌患者外周血CD4+T淋巴细胞中的异常表达及其相关机制研究[J].重庆医学,2017,46(33):4667-4669,4672. [9] 窦伟,李艳娇,张杰,等.舒肝宁注射液联合还原型谷胱甘肽对原发性肝癌经导管动脉化疗栓塞术后患者肝功能和血清T淋巴细胞水平的影响[J].中国医药,2018,13(9):1349-1352. [10] 郭存丽,毕玉美,刘钊,等.肝细胞癌患者外周血死亡受体表达及其与CD8+T淋巴细胞凋亡的相关性[J].中华肝脏病杂志,2018,26(2):125-129. [11] Sengsayadeth S, Wang T, Lee SJ, et al. Cytotoxic T-lymphocyte antigen-4 single nucleotide polymorphisms are not associated with outcomes after unrelated donor transplantation: A center for international blood and marrow transplant research analysis[J].Biol Blood Marrow Transplant,2014,20(6):900-903. [12] Jrgensen KW, Rasmussen M, Buus S, et al. NetMHCstab - Predicting stability of peptide-MHC-I complexes; impacts for cytotoxic T lymphocyte epitope discovery[J].Immunology,2014,141(1):18-26.
2020, Vol. 37 
