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| Effect of Runx2 Overexpression Lentivirus on Proliferation and Osteogenesis of Human Periodontal Ligament Cells in Vitro |
| HU Yan-he, HU Bai, LIU Jun |
| Department of Stomatology, Liyuan Hospital Affiliated to Tongji College of Huazhong University of Science and Technology, Wuhan Hubei, 430077 |
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Abstract 【Objective】To investigate the effect of Lentiviral-mediated Runx2 on proliferation and osteogenic potential of human periodontal ligament cells (PDLCs) in vitro.【Methods】PDLCs were cultured in vitro. The logarithmic cells were divided into blank control group (BC group, without any treatment), negative control group (NC group, infected cells without Runx2 gene with MOI = 50) and Runx2 group (transfected cells with Runx2 overexpression of MOI = 50). After 48 hours of transfection, the expression of GFP was observed under fluorescence microscope, and the transfection efficiency was calculated. MTT was used to detect cell proliferation in vitro, and real-time fluorescent quantitative PCR and Western blot were used to detect the expression of Runx2, BMP-2, ALP and OPN.【Results】After 48 hours of transfection, there was no green fluorescent protein in BC group, and a large number of cells in Runx2 group and NC group expressed green fluorescent protein. The transfection efficiency of Runx2 group and NC group was (88.57±3.07)% and (89.43±2.09)% respectively, there was no significant difference between the two groups (P>0.05). The odvalue of the number of living cells in Runx2 group was significantly higher than that in NC group and BC group at different time points, and the difference was statistically significant (mean P<0.05); the odvalue of the number of living cells in NC group and BC group was not statistically significant (P>0.05); the mRNA and protein expression of Runx2, BMP-2, ALP and OPN in Runx2 group were significantly higher than that in NC group and BC group, and the difference was statistically significant (all P<0.05); while in NC group There was no significant difference in the mRNA expression of Runx2, BMP-2, ALP and OPN between the two groups (P>0.05).【Conclusion】Runx2 overexpression of lentivirus can promote the proliferation of PDLCs in vitro and enhance their osteogenic ability, which has an important application prospect in periodontal tissue repair and regeneration.
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Received: 09 April 2019
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2020, Vol. 37 
