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| Detection and Correlation Analysis of CTHRC1, TGF-β1 and Smad7 Expression in Oral Submucous Fibrosis |
| WU Hao, LIU Jian, XIE Hui |
| Department of Periodontology, Changsha Stomatological Hospital , Changsha 410005, China |
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Abstract 【Objective】To investigate the expression of CTHRC1 (collagen triple helix repeat containing 1),TGF-β1 and smad7 in OSF (Oral Submucous Fibrosis) by immunohistochemistry in order to explore the correlation between CTHRC1 and TGF-β1/smad signaling pathway in pathogenesis of OSF. 【Methods】Buccal mucous tissues were obtained from 33 OSF patients (10 cases in the early stage, 13 cases in the middle stage and 10 cases in the late stage) and 10 healthy volunteers' mucous tissues. After paraffin embedding, the expression of CTHRC1,TGF-β1 and smad7 proteins in sections were detected by immunohistochemistry. The correlations between TGF-β1 expression and CTHRC1, smad7 were tested by Pearson software. 【Results】The expression of CTHRC1 in the early, middle and later stage OSF was significantly higher than that in normal oral mucosa(P=0.007,0.002,0.006). SO did the expression of smad7 in all stages of OSF(P=0.032,0.007,0.005). The expression of TGF-β1 was increased in the early and middle stage OSF tissues (P=0.007,0.031), however, there was no significant difference between the late expression and the normal control (P=0.323). There was a positive correlation between TGF-β1 and CTHRC1 expression in OSF lesions (R=0.678,P=0.0010), and there was no significant correlation between TGF-β1 and Smad7 expression (R=0.003,P=0.9908). 【Conclusions】The expression of CTHRC1 increases significantly in all stages of OSF,which is positively correlated with the expression of TGF-β1. CTHRC1 can be a new target for anti-fibrosis.
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Received: 23 December 2018
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2019, Vol. 36 
