|
|
|
| Imaging Vascular Networks on the Whole brain Scale Using High Resolution Synchrotron Radiation Technique |
| SHI Shu-peng, HU Xin-hang, FU Dong-ni,et al |
| Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China |
|
|
|
|
Abstract 【Objective】To explore the application of synchrotron radiation phase contrast imaging in the study of rat brain microvascular network.【Methods】Five SD rats were perfused without contrast medium to obtain brain, and then scanned and imaged based on synchrotron radiation phase contrast imaging technology. Multidimensional multi-plane reconstruction was performed using filtering algorithm and imaging software.【Results】A complete visualization system from projection map, two-dimensional slice to 3D image of whole cerebrovascular network was established. The skeleton of whole cerebrovascular network was further extracted and the rendered images of different color levels were obtained. 【Conclusion】The establishment of brain microvascular visualization system provides a new imaging basis for multi-plane and multi-angle analysis of 3D blood supply distribution in the whole brain and local target areas, and provides a powerful technical support for the study of cerebrovascular diseases.
|
|
Received: 19 April 2019
|
|
|
|
|
|
[1] Hayashi T, Deguchi K, Nagotani S,et al. Cerebral ischemia and angiogenesis[J].Curr Neurovasc Res,2006,3(2):119-129.
[2] Seevinck PR, Deddens LH, Dijkhuizen RM. Magnetic resonance imaging of brain angiogenesis after stroke[J].Angiogensis,2010, 13(2):101-111.
[3] Elliott J, Smith M. The acute management of intracerebral hemorrhage: a clinical review[J].Anesth Analg,2010, 110(5):1419-1427.
[4] Figueiredo G, Brockmann C, Boll H, et al. Comparison of digital subtraction angiography, micro-computed tomography angiography and magnetic resonance angiography in the assessment of the cerebrovascular system in live mice[J].Clin Neuroradiol,2012, 22(1): 21-28.
[5] Liu X, Zhao J, Sun J, et al. Lung cancer and angiogenesis imaging using synchrotron radiation[J].Phys Med Biol,2010, 55(8): 2399-2409.
[6] Hwu Y, Tsai WL, Chang H M, et al. Imaging cells and tissues with refractive index radiology[J].Biophys J,2004, 87(6):4180-4187.
[7] Yanev P, Dijkhuizen RM. In vivo imaging of neurovascular remodeling after stroke[J].Stroke,2012, 43(12): 3436-3441.
[8] Beck H, Plate KH. Angiogenesis after cerebral ischemia[J].Acta Neuropathol,2009, 117(5): 481-496.
[9] Plouraboue F, Cloetens P, Fonta C,et al. X-ray high-resolution vascular network imaging[J].J Microsc,2004, 215(Pt 2): 139-148.
[10] Heinzer S, Krucker T, Stampanoni M,et al. Hierarchical microimaging for multiscale analysis of large vascular networks[J].Neuroimage,2006, 32(2): 626-636. |
|
|
|
2019, Vol. 36 
