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| Effects of Rubescene B Combined with Capecitabine on Proliferation and Invasion of Breast Cancer Cells |
| ZHANG JUN-mei |
| Clinical Laboratory,Tangshan Luannan County Hospital,Hebei 063500 China |
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Abstract 【Objective】 To investigate the effect of rubescene B combined with capecitabine on proliferation and invasion of breast cancer cells.【Method】 The breast cancer cell line 4T1was established and randomly divided into four groups: rubescene B group (group A), capecitabine group (group B), rubescene B + capecitabine group (group C) and blank control group (group D). MTT assay was used to detect the increment inhibition rate of breast cancer cell line 4T1 in each group. The effect of drugs on invasive ability of breast cancer cell line 4T1 was determined by Transwell chamber assay. RT-PCR method was used to detect the expression of beta-catenin, cyclin D1 and c-myc mRNA in breast cancer cell line 4T1. 【Results】 Treatment in group A, B and C all inhibited the proliferation of breast cancer 4T1 cells, and group C had the strongest inhibition, which was higher than the other groups (P<0.05). The invasiveness of breast cancer 4T1 cells was inhibited in group A, group B and group C. The invasiveness of breast cancer 4T1 cells in group C was the weakest, which was lower than that of the other three groups (P<0.05). The expression of beta-catenin, cyclin D1 and c-myc was significantly down-regulated by rubescene B combined with capecitabine (P<0.05). 【Conclusions】 Rubescene B combined with capecitabine can significantly inhibit the proliferation of breast cancer 4T1 cells, and inhibit cell invasion, which is related to the suppressing the Wnt/β-catenin signaling transduction pathway.
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Received: 28 March 2018
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[1] 郭萍, 李玉山, 郭远强. 冬凌草化学成分和药理活性研究进展[J].药理评价研究,2010, 33 (2), 144-146.
[2] 胡雪, 董磊. 术前口服卡培他滨对直肠癌疗效及PTEN蛋白表达的影响[J].医学临床研究, 2016, 33(6):1205-1207.
[3] Ramirez RA, Beyer DT, Chauhan A,et al. The role of capecitabine/temozolomide in metastatic neuroendocrine tumors[J].Oncologist,2016, 21(6):671-675.
[4] 史晓贤, 李高文. 冬凌草乙素通过Wnt/β-catenin信号通路对裸鼠K562细胞移植瘤的抑制作用[J].新医学, 2018, 49 (1), 30-35.
[5] 王云飞, 顾政一, 聂勇战, 等.贝母素乙增强阿霉素对胃癌多药耐药裸鼠移植瘤的抑制作用及其机制研究[J].中草药, 2014, 45 (5), 686-690.
[6] Lu J, Chen X, Qu S, et al. Oridonin induces G2/M cell cycle arrest and apoptosis via the PI3K/Akt signaling pathway in hormone-independent prostate cancer cells[J].Oncol Lett,2017, 13(4):2838-2846.
[7] Prasad S,Gupta SC,Tyagi AK, et al. γ-Tocotrienol suppresses growth and sensitises human colorectal tumours to capecitabine in a nude mouse xenograft model by down-regulating multiple molecules[J].Br J Cancer,2016, 115(7):814-824.
[8] Van Zuylen WJ, Rawlinson WD, Ford CE. The Wnt pathway: a key network in cell signaling dysregulated by viruses[J].Rev Med Virol,2016, 26 (5), 340-355. |
| [1] |
. [J]. JOURNAL OF CLINICAL RESEARCH, 2019, 36(1): 145-147. |
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2019, Vol. 36 
