沙立芦单抗对类风湿关节炎滑膜细胞增殖和凋亡的影响

doi:10.3969/j.issn.1671-7171.2023.01.030

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Abstract 【Objective】 To investigate the effects of sarilumab on the proliferation and apoptosis of rheumatoid arthritis (RA) cells, and to analyze the related mechanisms. 【Methods】 Human RA cell line FLS cells (RA-FLS cells) were cultured in vitro and divided into the control group, the sarilumab low, medium and high dose groups (final concentration of 2.5 mg/L, 5.0 mg/L and 10.0 mg/L, respectively). The proliferation inhibition rate of RA-FLS cells was detected by CKK-8 and the apoptosis rate of RA-FLS cells was detected by Annexin V-FITC/PI double staining. The expression of anti-proliferation related nuclear Antigen (PCNA) , apoptosis protein B lymphocyte-2 (Bcl-2), Bcl-2 related X protein (Bax) and protein tyrosine kinase of Janus kinase 1/STAT3 pathway protein (JAK1, p-JAK1, STAT3 and p-STAT1) was detected by Western blotting. 【Results】 The proliferation inhibition rate, apoptosis rate and expression levels of PCNA and Bcl-2 protein in RA-FLS cells from the sarilumab low, middle and high dose groups were higher than those in the control group (P<0.05), while the levels of Bax and p-JAK1/JAK1, p-STAT3/STAT3 were significantly lower than those in the control group (P<0.05). All of those dectections were in dose dependent manner (P<0.05).【Conclusion】 Sarilumab may inhibit the proliferation of RA-FLS cells and induce apoptosis by inhibiting the activation of IL-6/JAK1/STAT3 pathway.

Key words： Arthritis, Rheumatoid Antibodies, Monoclonal Synoviocytes Cell Proliferation Apoptosis

Received: 27 January 2022

PACS:

R593.22

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	WANG Xiao
	WANG Gui-ling
	LIU Tong

Cite this article:

WANG Xiao,WANG Gui-ling,LIU Tong. Effect and Mechanism of Sarilumab on Proliferation and Apoptosis of Rheumatoid Arthritis Synoviocytes[J]. JOURNAL OF CLINICAL RESEARCH, 2023, 40(1): 102-104.

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http://journal07.magtech.org.cn/yxlcyj/EN/10.3969/j.issn.1671-7171.2023.01.030 OR http://journal07.magtech.org.cn/yxlcyj/EN/Y2023/V40/I1/102

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