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| Level and Significance of SIRT7 in Peripheral Blood Mononuclear Cells of Patients with Non-alcoholic Fatty Liver Disease |
| XIN Jie-jing, XU Juan, SHEN Jie, et al |
| Department of infectious diseases,Affiliated Hospital of Yan'an University,Yan'an Shaanxi 716000 |
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Abstract 【Objective】To investigate the level and significance of silent information regulator 7 (SIRT7) in peripheral blood mononuclear cells of patients with non-alcoholic fatty liver disease (NAFLD). 【Methods】A total of 205 patients with NAFLD (the NAFLD group) were selected as the research subjects from three hospitals.Healthy volunteers who participated in the health examination at the same time were selected as the control group (n=100). Ficoll density gradient centrifugation was used to isolate mononuclear cells in peripheral blood; Western blot was used to detect the expression level of SIRT7 in mononuclear cells. The relationship between SIRT7 and the severity of NAFLD was analyzed. 【Results】The expression level of SIRT7 in the NAFLD group was lower than that in control group,the difference was statistically significant (P<0.05). With the aggravation of NAFLD,the expression level of SIRT7 gradually decreased. The area under ROC curve,sensitivity and specificity of SIRT7 in the diagnosis of moderate and severe NAFLD were 0.890,75.61% and 87.80%,respectively. Logistic regression analysis showed that FPG,TC,TG,ALT,AST,and SIRT7 were all independent risk factors for progression to moderate and severe NAFLD (P<0.05). The predicted curve constructed by FPG,TC,TG,ALT,AST,and SIRT7 had a high degree of coincidence between the fit curve of the nomogram model and the ideal curve with a C-index of 0.963. 【Conclusion】The low expression level of SIRT7 in peripheral blood mononuclear cells of NAFLD patients indicates that the disease is serious. The nomogram models constructed by FPG,TC,TG,ALT,AST,and SIRT7 have high discrimination and precision,and can effectively judge moderate and severe NAFLD.
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Received: 08 March 2022
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[1] REZAEI TAVIRANI M,REZAEI TAVIRANI M,ZAMANIAN AZODI M. ANXA2,PRKCE,and OXT are critical differentially genes in nonalcoholic fatty liver disease[J].Gas Hep Bed Bench,2019,12(2):131-137.
[2] 刘新.SIRT7生物学功能与人类疾病研究进展[J].济宁医学院学报,2017,40(4):295-300.
[3] ZHANG S,CHEN P,HUANG Z,et al. Sirt7 promotes gastric cancer growth and inhibits apoptosis by epigenetically inhibiting miR-34a[J].Sci Rep,2015,5:9787.
[4] ARAKI S,IZUMIYA Y,ROKUTANDA T,et al. Sirt7 contributes to myocardial tissue repair by maintaining transforming growth factor-β signaling pathway[J].Circulation,2015,132(12):1081-1093.
[5] 中国研究型医院学会肝病专业委员会,中国医师协会脂肪性肝病专家委员会,中华医学会肝病学分会脂肪肝与酒精性肝病学组,等.中国脂肪性肝病诊疗规范化的专家建议(2019年修订版)[J].中华肝脏病杂志,2019,27(10):748-753.
[6] LI L,LIU D W,YAN H Y,et al. Obesity is an independent risk factor for non-alcoholic fatty liver disease:evidence from a meta-analysis of 21 cohort studies[J].Obes Rev,2016,17(6):510-519.
[7] TANG X,WANG J,XIA X,et al. Elevated expression of ciRS-7 in peripheral blood mononuclear cells from rheumatoid arthritis patients[J].Diagn Pathol,2019,14(1):11.
[8] YE G,QIN Y,WANG S,et al. Lamc1 promotes the warburg effect in hepatocellular carcinoma cells by regulating PKM2 expression through AKT pathway[J].Cancer Biol Ther,2019,20(5):711-719.
[9] 王彦丽,郝礼森,张铁强,等.非酒精性脂肪性肝病患者外周血T细胞亚群的变化及意义[J].实用预防医学,2018,25(12):1473-1475.
[10] SHIN J,HE M,LIU Y,et al. SIRT7 represses myc activity to suppress ER stress and prevent fatty liver disease[J].Cell Rep,2013,5(3):654-665.
[11] RYU D,JO Y S,LO SASSO G,et al. A SIRT7-dependent acetylation switch of GABPβ1 controls mitochondrial function[J].Cell Metab,2014,20(5):856-869.
[12] XU Y,GUAN Y,YANG X,et al. Association of serum homocysteine levels with histological severity of NAFLD[J].J Gastrointestin Liver Dis,2020,29(1):51-58.
[13] 苑喜微,李冬冬,刘领弟,等.血红素氧合酶1在非酒精性脂肪性肝病诊断中的应用研究[J].中华肝脏病杂志,2019,27(4):291-297. |
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2022, Vol. 39 
