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| Relationship between the Methylation Degree of RASSF1A and WIF-1 Genes and the Efficacy of Neoadjuvant Radiotherapy and Chemotherapy in Rectal Cancer Patients |
| ZHANG Rong-zhi, JING Wei |
| Anorectal Hospital,Ankang Hospital of Traditional Chinese Medicine,Ankang Shaanxi 725000 |
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Abstract 【Objective】 To investigate the relationship between the methylation of Ras related region family 1A gene (RASSF1A) and Wnt inhibitor factor-1(WIF-1) gene in serum and the efficacy of neoadjuvant radiotherapy and chemotherapy (nCRT) in patients with rectal cancer. 【Methods】A total of 109 patients with rectal cancer in our hospital were selected as the study subjects,and all of them were treated with nCRT. The treatment effect was analyzed. The patients were divided into observation group (nCRT sensitive,n=73) and control group (nCRT insensitive,n=36) according to the tumor regression grade (TRG) standard; The age,sex,tumor distance from anal position,differentiation type,clinical stage,lymph node metastasis and associated diseases of the two groups were collected,and the positive expression rates of RASSF1A and WIF-1 gene methylation in the two groups were compared,the correlation between the methylation degree of RASSF1A and WIF-1 genes and the efficacy of nCRT in rectal cancer patients were analyzed. 【Results】 There was no significant difference between the two groups in age,sex,distance from the tumor to the anal position,differentiation type,clinical stage,lymph node metastasis,combined disease constituent ratio,blood pressure and blood lipid level (P>0.05). The positive rates of RASSF1A gene and WIF-1 gene methylation in serum of the observation group were 16.44% (12/73) and 13.70% (10/73),both lower than 41.67% (15/36) and 33.33% (12/36) of the control group (P<0.05). Spearman test showed that the methylation degree of RASSF1A and WIF-1 genes in serum was negatively correlated with the curative effect (P<0.01). 【Conclusion】The degree of methylation of RASSF1A and WIF-1 genes is closely related to the efficacy of NCRT in patients with rectal cancer. The higher the positive rate of methylation of RASSF1A and WIF-1 genes,the lower the efficacy of NCRT in patients with rectal cancer.
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Received: 10 January 2022
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2022, Vol. 39 
