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| Effects of Interfering with PARP1 Gene Expression on Proliferation, Invasion and Migration of Gastric Cancer MGC-803 Cells |
| CAO Qiu-xiang, NAN Hong, PAN Xiao-tao |
| Baoji Caijiapo Hospital,Baoji Shaanxi,722405 |
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Abstract 【Objective】 To investigate the effect of poly adenosine diphosphate ribose polymerase 1 (PARP1) gene on the proliferation, migration and invasion of gastric cancer cell line (MGC-803).【Methods】 Human gastric cancer cell line MGC-803 and human normal gastric mucosal epithelial cell GES-1 were cultured in vitro, and the expression of PARP1 mRNA was compared between the two cells; MGC-803 cells were randomly divided into control group, interference PARP1 expression group (si-PARP1 group) and negative control group (si-NC group); The expression of PARP1 mRNA in MGC-803 cells was detected by qRT-PCR; MTT assay, flow cytometry, scratch test and Transwell chamber method were used to detect cell proliferation, apoptosis, migration and invasion respectively. Western blot was used to detect the expression of PARP1, matrix metalloproteinase-2 (MMP-2), MMP-9, RhoA and Rock1 proteins. 【Results】 Compared with GES-1, the expression of PARP1 mRNA in MGC-803 cells increased significantly (P<0.05); Compared with the control group and the si-NC group, the apoptosis rate and proliferation inhibition rate of MGC-803 cells in the si-PARP1 group increased significantly (P<0.05), and the migration rate and invasion rate of cell scratch, the expression levels of PARP1 mRNA and PARP1 protein, MMP-2, MMP-9, RhoA and Rock1 protein decreased significantly (P<0.05). 【Conclusion】 Interfering with the expression of PARP1 can inhibit the proliferation, migration and invasion of human gastric cancer cells, which may be related to the inhibition of RhoA/Rock signaling pathway.
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Received: 10 December 2021
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2022, Vol. 39 
