|
|
|
| Expression and Clinical Significance of Serum MiR-98 and IL-13 in Elderly Patients with Acute Attack of Asthma |
| LI Chao, ZHANG Jun-yan |
| Department of Respiratory and Critical Care Medicine, Ankang Central Hospital, Ankang Shaanxi 725000 |
|
|
|
|
Abstract 【Objective】To investigate the expression level and clinical significance of serum microrna-98(miR-98) and interleukin-13(IL-13) in elderly patients with acute attack of asthma. 【Methods】A total of 360 elderly patients with asthma diagnosed in the Ankang Central Hospital from March 2017 to January 2020 was selected, including 200 cases in the acute attack group (90 cases in the mild group, 80 cases in the moderate group and 30 cases in the severe group) and 160 cases in the remission group; In addition, 90 elderly people who underwent physical examination in the Ankang Central Hospital in the same period were selected as the health group. The relative expression of serum miR-98 was detected by real-time fluorescence quantitative PCR (Quantitative Real-time PCR,qRT-PCR), the level of serum IL-13 was detected by enzyme-linked immunosorbent assay, and the first second forced expiratory volume (FEV1) and maximum peak expiratory flow rate (PEF) in acute attack group were detected and compared by pulmonary function instrument. 【Results】 The expression of serum miR-98 in the acute attack group was significantly lower than that in the remission group and the healthy group, and decreased successively with the aggravation of acute attack (P<0.05); The expression of serum IL-13 was higher than that in the remission group and the healthy group, and increased successively with the aggravation of acute attack (P<0.05). FEV1 and PEF in the mild group, the moderate group and the severe group decreased successively with the aggravation of the disease(P<0.05). The relative expression of miR-98 in serum of patients with the acute attack group was positively correlated with FEV1 and PEF(P<0.05), and negatively correlated with IL-13(P<0.05); The expression level of IL-13 in serum was negatively correlated with FEV1 and PEF (P<0.05).【Conclusion】The abnormal expression of serum miR-98 and IL-13 in patients with acute attack of asthma is related to the degree of attack and the indexes of pulmonary function FEV1 and PEF. It can be used as a potential index to evaluate the elderly asthma.
|
|
Received: 06 December 2021
|
|
|
|
|
|
[1] 李文娟,吴超杰,吉宁飞,等. 老年支气管哮喘病人控制现状及影响因素[J].实用老年医学,2017,31(7):630-632.
[2] DUNN R M. Asthma in the elderly and late-onset adult asthma[J].Allergy,2018,73(2):284-294.
[3] 蒋倩.同伴支持教育对支气管哮喘患者自我管理水平及症状控制的影响[J].医学临床研究,2020,37(7):1067-1069.
[4] 吴峰妹,苏士成,李红,等. 支气管哮喘急性发作期气道炎症表型与中医辨证分型的关系[J].浙江中西医结合杂志,2020,30(5):426-428.
[5] 吴迪. 支气管哮喘气道重塑发生机制及其中西医防治现状[J].辽宁中医药大学学报,2019,21(5):107-110.
[6] 孔晓梅,韩葆芬,张彩苹,等. 支气管哮喘急性发作期的病原学分析[J].中华医院感染学杂志,2014,24(1):58-60.
[7] 李雪静,张园园. miRNA调控支气管哮喘研究进展[J].临床儿科杂志,2019,37(6):466-469.
[8] 肖雪清. miR-98在哮喘患儿外周血单个核细胞中的表达及意义[J].中国小儿急救医学,2017,24(3):201-204.
[9] 中华医学会呼吸病学分会哮喘学组. 支气管哮喘防治指南(2020年版)[J].中华结核和呼吸杂志,2020,43(12):1023-1048.
[10] 沈美珠,夏元旦,周敏,等. 老年支气管哮喘患者临床表现及炎症特征分析[J].老年医学与保健,2018,24(2):191-194.
[11] MIDYAT L,GULEN F,KARACA E,et al. MicroRNA expression profi-ling in children with different asthma pfenotypes[J].Pediatr Pulm,2015,6(10):75-79.
[12] 戴春威,张燕妮,刘倩. 支气管哮喘患儿血清miR-98-5p及IL-13的表达水平及临床意义[J].内科,2017,12(4):484-487.
[13] 钱慧,钱文霞. 支气管哮喘患者急性发作期外周血LncRNA NEAT1表达及与Th1/Th2平衡的关系[J].内科急危重症杂志,2020,26(5):33-36.
[14] 冯海燕,谭建新. IL-4、IL-5和IL-13与哮喘发生发展的关系[J].心理医生,2017,23(3):119-120.
[15] 李燚,李蓉,贾可,等. 血清微小RNA Let-7a表达水平对儿童哮喘诊断及严重程度评估的价值[J].国际检验医学杂志,2020,41(9):1078-1081.
[16] 魏淑丽.支气管哮喘患儿血清中Eotaxin、IL-13的表达水平及临床意义[J].川北医学院学报,2018,33(2):206-209.
[17] 赵燕平,关键民,赵玉娟. 慢性阻塞性肺疾病继发肺动脉高压患者外周血微小RNA-98的表达水平及临床意义[J].临床内科杂志,2019,36(5):321-324. |
|
|
|
2022, Vol. 39 
