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| Expressions and Significance of UHRF-1 and DNMT-1 in Tissues of Gastric Cancer |
| XIAO Qin-rong, ZHU Wei-fang, DA Rong-feng, et al |
| First Peoples Hospital of Tianmen City, Hubei Province,431700 |
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Abstract 【Objective】 To investigate the expressions of UHRF-1 (ubiquitin-like PHD with ring finger domain-1) and DNMT-1 (DNA methyltransferase-1) in gastric cancer and its relationship with clinicopathological features. 【Methods】A total of 75 gastric cancer patients treated in our hospital were selected as the research subjects. The lesion tissues and para-cancerous tissues were collected. The mRNA expressions of UHRF-1, DNMT-1 were detected by RT-PCR and immunohistochemistry, and the relationship between expression and the clinicopathological characteristics were analyzed. 【Results】The protein positive rates of UHRF-1, DNMT-1 in gastric cancer tissues were 84% (63/75) and 70.7% (53/75), respectively, which were significantly higher than those in the para-cancerous tissues 45% (34/75) and 20% (18/75), respectively (P<0.05). The mRNA expression levels of UHRF-1, DNMT-1 in gastric cancer tissues were significantly higher than those of para-cancerous tissues (P<0.05). The protein positive rates of UHRF-1 and DNMT-1 in gastric cancer tissues with tumor size (≥3 cm), moderately or poorly differentiated carcinomas, serosal invasion, stage Ⅲ+Ⅳ and lymphatic metastasis were higher than that of tumors <3 cm, well-differentiated carcinomas, none serosal infiltration, stage Ⅰ+Ⅱ, and no lymphatic metastasis. The difference was significant (P<0.05).【Conclusion】The expressions of UHRF-1and DNMT-1 in the lesion tissues of gastric cancer patients are significantly increased, and they are closely related to the clinicopathological features of patients with gastric cancer.
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Received: 20 May 2020
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[1] 张战胜, 徐东为. UHRF-1蛋白在胃癌组织中表达及其与胃癌患者临床病理特征关系[J].临床误诊误治, 2017, 30(5):89-93.
[2] TOSHINORI H, DANIEL J,WEISENBERGER P E. Genome-scale analysis of aberrant DNA methylation in colorectal cancer[J].Genome Res,2012, 22(2): 271-282.
[3] ZHANG J, GAO Q, LI P, et al. S phase-dependent interaction with DNMT-1 dictates the role of UHRF1 but not UHRF2 in DNA methylation maintenance[J].Cell Res,2011, 21(12):1723-1739.
[4] KWON M J, YUN M J, KIM M K. A female adnexal tumor of probable Wolffian origin showing positive O-6-methylguanine-DNA methyltransferase methylation [J].Obstet Gynecol Sci,2016, 59(4): 328-332.
[5] 史光军, 鲍建昌, 陈增银,等. 维甲酸受体α和β在胰腺癌组织中的表达及意义[J].中国现代普通外科进展, 2014, 17(11):872-876.
[6] 刘爽.P16^INK4A及Ki-67在宫颈腺癌组织中的表达及意义[J].解放军医学杂志, 2012, 37(3):225-228.
[7] 张伟, 周林, 李乃义, 等. 胃癌组织UHRF-1表达与预后相关性分析[J].中华肿瘤防治杂志, 2015, 22(2): 109-112.
[8] 周燚, 秦俭, 李旭,等. DNMT-1在胃癌组织中的表达及其对胃癌细胞增殖和迁移的影响[J].上海交通大学学报(医学版), 2016, 36(7):956-961. |
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2021, Vol. 38 
