|
|
|
| Expression and Significance of Phosphatidylinositol Kinase-3 and Protein Kinase Akt in Mkeletal Muscle of Postmenopausal Hip Fracture Patients |
| Mirarimu Multiza, LIN Hai-Shan, Eniwalj Da Maura, et al |
| Department of Orthopedic, Joint and Geriatric Osteopathy,The People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi City, Xinjiang Uygur Autonomous Region, 830001 |
|
|
|
|
Abstract 【Objective】 To investigate the expression levels and clinical significance of phosphatidylinositol kinase-3 (PI3K) and protein kinase (Akt) in skeletal muscle of postmenopausal hip fracture patients. 【Methods】 Prospectively selected a total of 80 post-menopausal patients who underwent surgery for hip fracture in our department from January 2017 to June 2019. Patients were divided into 4 different groups according to the dual-energy X-ray bone density (DXA) levels. They were normal bone mass group (-1≤T≤1, group A), bone mass reduction group (-2.5 <T <-1, group B), osteoporosis Group (T ≤-2.5, group C) and severe osteoporosis group(T ≤-2.5 with one or more brittle fractures, group D).A small amount of muscle tissue was taken from the lateral femoral muscle during the operation for RNA extract, quantitative Real-time PCR (RT-PCR) was detected for mRNA expression level of phosphatidyl inositol kinase- 3(PI3K)and protein kinase (Akt), while the protein expression levels of PI3K and Akt were measured by immunohistochemical technique. The correlation of PI3K and Akt with the bone density was analyzed as well. 【Results】 Both mRNA and protein expression levels of Akt and PI3K in the group D were higher than those in other groups (P<0.05).The mRNA and protein expression levels of Akt and PI3K in the group C was higher than that that in the group B (P<0.05). However, the mRNA and protein expression levels of Akt and PI3K were not statistically significant difference between the group A and B (P>0.05). The expression levels of AKT and PI3K were positively correlated with the bone mineral density of the patients. 【Conclusion】 The PI3K/Akt signaling pathway is activated in skeletal muscle of postmenopausal osteoporosis patients, and the mRNA and protein expression levels of PI3K and Akt are increased and also positively correlated with the degree of osteoporosis. It suggests that PI3K/Akt plays an important role in the regulation of bone-muscle formation and reconstruction.
|
|
Received: 13 January 2020
|
|
|
|
|
|
[1] 娄东辉,尹福在.骨质疏松性骨折风险评估研究进展[J].医学临床研究,2013,4(30):785-787.
[2] Cruzjentoft AJ,Baeyens JP,Bauer JM,et al.Sarcopenia: European consensus on definition and diagnosis: report of the European working group on sarcopenia in older people[J].Age Ageing,2010,39(4):412-423.
[3] 辜彦.高危老年骨质疏松症患者骨代谢生化指标检测的临床意义[J].医学临床研究,2016,33(11):2157-2159.
[4] 陈亚辉, 龚忠勤,崔燎.PI3K /Akt信号通路在骨质疏松病理过程中的作用[J].中国骨质疏松杂志,2015,3(21):356-359.
[5] Rosen CJ.美国骨矿研究学会骨矿盐疾病与代谢性骨病学[M].北京: 北京大学医学出版社,2014.
[6] Christian MG.Integrated therapies for osteoporosis and sarcopenia: from signaling pathways to clinical trials[J].Calcif Tissue Int,2015,96(3):243-255.
[7] 中华医学会骨质疏松和骨矿盐疾病分会. 原发性骨质疏松症诊疗指南[J].中华骨质疏松和骨矿盐疾病杂志,2017,9(10): 413-416.
[8] Curtis E,Litwic A,Cooper C ,et al.Determinants of muscle and bone aging[J].J Cell Physiol,2015,230(11):2618-2625.
[9] Tagliaferri C, Wittrant Y, Davicco MJ, et al. Muscle and bone, two interconnected tissues [J].Ageing Res Rev,2015, 21: 55-70.
[10] 王秉义,潘剑.丹参素拮抗氧化应激所致骨质疏松并通过PI3k /Akt通路减少成骨细胞的凋亡[J].中国骨质疏松杂志,2017,1(23):1-6.
[11] Hayakawa N, Fukumura J, Yasuno H, et al.1α,25(OH)2D3 downregulates gene expression levels of muscle ubiquitin ligases MAFbx and MuRF1 in human myotubes [J].Biomed Res,2015,36 (2):71-80.
[12] Shiotani A,Takami M,Itoh K,et al. Regulation of osteoclast differentiation and function by receptor activator of NFk B ligand and osteoprotegerin[J].Anat Rec,2002,268 (2):137-146.
[13] Pawlak J, Derlacz RA.The mechanism of insulin resistance in peripheral tissues[J].Postepy Biochem,2011, 57(2):200-206. |
| [1] |
. [J]. JOURNAL OF CLINICAL RESEARCH, 2019, 36(11): 2259-2260. |
|
|
|
|
2020, Vol. 37 
