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| Effect of siRNA Targeting UHRF1 on Proliferation and Apoptosis of HeLa Cells |
| GAO Yan-fang, ZHANG Ming, FAN Ju-xiang, et al |
| Changzhou Maternal and Child Health Hospital Affiliated to Nanjing Medical University, Jiangsu Changzhou 213000 |
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Abstract 【Objective】 To investigate the effects of ubiquitin-like containing PHD and ring finger 1 (UHRF1) gene silencing by specific small interfering RNA (siRNA) on the proliferation and apoptosis in human cervical cancer HeLa cells in vitro.【Methods】 HeLa cells were randomly divided into three groups: the untreated control group without transfection, the negative control group with scramble siRNA transfection and the silencing group which were transfected with the UHRF1 specific gene-targeted siRNA. The mRNA and protein levels of UHRF1 were detected by RT-PCR and western blot, respectively. The effects of UHRF1 gene silencing on the proliferation and apoptosis were analyzed by conducting CCK-8 assay and flow cytometry with Annexin V-FITC/PI staining. 【Results】 The RT-PCR and western blot results showed the mRNA and protein levels of UHRF1 were significantly decreased in HeLa cells after the transfection of UHRF1 gene-targeted siRNA (P<0.01). The proliferation was inhibited and apoptosis was triggered via silencing UHRF1 gene, and the difference between the silencing group and the untreated control group/ negative control group was statistically significant (P<0.01). However,There was no significant difference between the untreated control group and the negative control group (P>0.05). 【Conclusion】 The expression of UHRF1 gene was efficiently interfered by transfecting the UHRF1-targeted siRNA, which resulted in decreased proliferation and enhanced apoptosis in HeLa cells.
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Received: 10 July 2019
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[1] Bray F, Ferlay J, Soerjomataram I,et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J].CA Cancer J Clin,2018, 68(6): 394-424.
[2] 吴敏, 龚颖萍, 程卫, 等. 宫颈癌机会性筛查176919例临床分析[J].医学临床研究, 2018, 35(7):1345-1346.
[3] Babbio F, Pistore C, Curti L, et al. The SRA protein UHRF1 promotes epigenetic crosstalks and is involved in prostate cancer progression [J].Oncogene,2012, 31(46): 4878.
[4] Wang F, Yang YZ, Shi CZ, et al. UHRF1 promotes cell growth and metastasis through repression of p16 ink4a in colorectal cancer [J].Ann Surg Oncol,2012, 19(8): 2753-5762.
[5] Ge TT.UHRF1 gene silencing inhibits cell proliferation and promotes cell apoptosis in human cervical squamous cell carcinoma CaSki cells [J].J Ovarian Res,2016, 9(1): 42.
[6] 黄鑫, 姜继勇, 刘立立, 等. UHRF1在宫颈癌组织中的表达及其临床意义 [J].临床肿瘤学杂志, 2018, 23(2): 137-141.
[7] Lu H, Hallstrom TC. The nuclear protein UHRF2 is a direct target of the transcription factor E2F1 in the induction of apoptosis [J].J Biol Chem,2013, 288(33): 23833-23843.
[8] Guan D, Factor D, Liu Y, et al. The epigenetic regulator UHRF1 promotes ubiquitination-mediated degradation of the tumor-suppressor protein promyelocytic leukemia protein [J].Oncogene,2013, 32(33): 3819.
[9] Rothbart SB, Krajewski K, Nady N, et al. Association of UHRF1 with methylated H3K9 directs the maintenance of DNA methylation [J].Nat Struct Mol Biol,2012, 19(11): 1155-1160. |
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. [J]. JOURNAL OF CLINICAL RESEARCH, 2020, 37(4): 598-600. |
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2020, Vol. 37 
