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| Effect of Curcumol on Proliferation and Migration of Mouse Melanoma B16 Cells |
| NING Ning, JIANG Yu, ZOU Lian-hong, et al |
| Medical department of Hunan provincial people's hospital, Changsha 410005,Hunan |
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Abstract 【Objective】To investigate the effect and mechanism of curcumol on proliferation and migration of mousemelanoma B16 cell. 【Methods】B16 cells were cultured in RPMI-1640 medium with 10% fetal bovine serum. The proliferation, migration and mir-7-5p content of B16 cells treated with Curcumol (50, 100, 200μmol / L) were detected. B16 cells were pretreated with mir-7-5p inhibitor, then treated with Curcumol (100 μ mol / L) to detect cell proliferation and migration.【Results】The 490 nm absorbance of B16 cells treated with different doses of Curcumol (50, 100, 200μmol / L) was lower than that of the control group, and with the increase of Curcumol Concentration, the 490 nm absorbance of the cells decreased significantly, the difference was statistically significant (P<0.05). After treatment of B16 cells with different doses of Curcumol (50, 100, 200 μ mol / L), the number of transmembrane cells was lower than that of the control group, and with the increase of Curcumol Concentration, the number of transmembrane cells decreased significantly, the difference was statistically significant (P<0.05).The inhibitory effect of miR-7-5p inhibitor on B16 cells was verified by real-time PCR. After NC inhibitor and miR-7-5p inhibitor were transfected, the miR-7-5p of the cells were (1.02±0.10) and (0.41±0.14), respectively, with statistical significance (P<0.05). After inhibiting miR-7-5p of B16 cells, the regulation of Curcumol on cell proliferation was observed, compared with NC inhibitor (1.413) and the difference was statistically significant (P<0.05). Compared with NC inhibitor (1.413±0.108), miR-7-5p inhibitor increased the absorbance value (1.697±0.089) of B16 cells treated with Curcumol,the difference was statistically significant (P<0.05).【Conclusion】Curcumol could inhibit cell proliferation and migration of mouse melanoma B16 cells, and its mechanism might be related to the increased expression of miR-7-5p.
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Received: 21 May 2019
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2020, Vol. 37 
