大鼠血清骨形成标志物与骨骼纵向生长关系的实验研究

doi:10.3969/j.issn.1671-7171.2019.04.027

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Abstract 【Objective】 To investigate the relationship between serum bone formation markers and bone longitudinal growth. 【Methods】Forty-seven clean male SD rats aged 3 weeks were randomly divided into dexamethasone group (27 rats) and control group (20 rats). The rats in the dexamethasone group were intraperitoneally injected with dexamethasone daily, while the rats in the control group were intraperitoneally injected with saline of equal volume for 10 days. The nasal tail length, body weight and tibial length of rats in the two groups were measured. Tibial tissue with growth plate was prepared and the total thickness of growth plate, the thickness of growth plate proliferation zone and the thickness of growth plate hypertrophy zone were measured. The levels of serum bone formation markers [osteocalcin (OC), I collagen carboxyl terminal propeptide (PICP) and alkaline phosphatase (ALP)] were measured by ELISA method in two groups of rats.【Results】The body weight, nasal tail length and tibia length of rats in the dexamethasone group were significantly lower than those in the control group (P<0.05); the thickness of growth plate, proliferative zone and hypertrophic zone of rats in the dexamethasone group were significantly lower than those in the control group (P<0.05); there were no significant differences in serum OC, ALP and PICP levels between the two groups (P>0.05). In the dexamethasone group, serum OC was positively correlated with tibial length and thickness of proliferative zone (P<0.05), serum ALP was negatively correlated with tibial length (P<0.05), and serum PICP was positively correlated with nasal tail length (P<0.05). 【Conclusion】Glucocorticoids can significantly inhibit the longitudinal growth of rat skeleton. OC, ALP and PICP may be potential indicators of the longitudinal growth of rat skeleton.

Key words： Rats Sprague-Dawley/GD Osteogenesis

Received: 24 October 2018

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R-332

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http://journal07.magtech.org.cn/yxlcyj/EN/10.3969/j.issn.1671-7171.2019.04.027 OR http://journal07.magtech.org.cn/yxlcyj/EN/Y2019/V36/I4/702

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