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| Effect of Sox9 Gene Expression on the Proliferation of Glioma Cells |
| GAO Lei, FU Wen-sheng, NIE Ben,et al |
| Hiser Medical Center of Qingdao, Qingdao 266033 |
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Abstract 【Objective】To investigate the effect of testis determinant HMG box 9 (Sox9) gene expression on the proliferation of glioma cells.s. 【Methods】The brain glioma U87 cells were selected and randomly divided into control group, blank transfection group and interference group. The blank transfection group was transfected with lentivirus, the interference group was transfected with Sox9 lentivirus. MTT method was used to detect cell proliferation, DAPI nuclear staining was used to detect cell apoptosis, real-time fluorescent quantitative PCR was used to detect Sox9 mRNA expression, Western blot was used to detect Sox9, Sox2, nestin protein expression.【Results】The expression of Sox9 mRNA in the interference group was significantly lower than that in the blank transfection group and the control group, the blank transfection group was significantly lower than that in the control group (P<0.05), the apoptosis rate in the interference group was significantly higher than that in the blank transfection group and the control group, and the blank transfection group was significantly higher than that in the control group (P<0.05). The OD value of the interference group at 48 h and 72 h was significantly lower than that of the blank transfection group and the control group, and that of the blank transfection group at 48 h and 72 h was significantly lower than that of the control group (P<0.05). The relative expression of Sox9, Sox2 and nestin protein in the interference group was significantly lower than that in the blank transfection group and the control group, while the relative expression of Sox9 and sox27 protein in the blank transfection group was significantly lower than that in the control group (P<0.05).【Conclusion】Sox9 gene expression can inhibit the proliferation of glioma cells, promote apoptosis and down regulate the expression of Sox2 and nestin protein.
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Received: 30 October 2018
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2019, Vol. 36 
