毛细管离心法在慢性再生障碍性贫血输血后患者RhCcEe血型鉴定中的应用价值

doi:10.3969/j.issn.1671-7171.2025.10.023

Abstract
Figure/Table
References (0)
Related Citation (15)

Download: PDF (1214 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract 【Objective】To investigate the significance of the capillary centrifugation method in identification of RhCcEe blood group in patients with chronic aplastic anemia (CAA) after blood transfusion.【Methods】 A total of 103 post-transfusion CAA patients were tested for RhCcEe blood group using three methods: microcolumn gel card method, capillary centrifugation method, and improved capillary centrifugation method. The results were compared with those of the polymerase chain reaction-sequence specific primer (PCR-SSP) method.【Results】 In RhCcEe blood group detection, the detection rate of the improved capillary centrifugation method was 71.84% (74/103), which was higher than that of the capillary centrifugation method (46.60%, 48/103) and the microcolumn gel card method (18.45%, 19/103), with statistically significant differences (P<0.05). The detection rate of the capillary centrifugation method was higher than that of the microcolumn gel card method (P<0.05). Among CAA patients, the detection rate of RhCcEe blood group by the improved capillary centrifugation method was 25.00% in those with transfusion interval <14 days, which was lower than those with transfusion interval of 14-28 days (75.29%) and interval >28 days (80.00%) (P<0.05). No significant difference was found between patients with intervals of 14-28 days and those with >28 days (P>0.05).【Conclusion】In RhCcEe blood group identification of CAA patients, the improved capillary centrifugation method shows a relatively higher detection rate. Moreover, the detection rate increases with the extension of the transfusion interval, making it suitable for rapid auxiliary diagnosis of RhCcEe blood group in clinical practice.

Key words： Anemia, Aplastic Chronic Disease Ultracentrifugation Blood Grouping and Crossmatching Blood Transfusion Diagnosis, Differential

Received: 08 December 2023

PACS:

R556.5

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	CHEN Xiaohong
	LI Dan
	LU Lijun

Cite this article:

CHEN Xiaohong,LI Dan,LU Lijun. Significance of Capillary Centrifugation Method in Identification of RhCcEe Blood Group in Patients with Chronic Aplastic Anemia after Transfusion[J]. JOURNAL OF CLINICAL RESEARCH, 2025, 42(10): 1760-1762.

URL:

http://journal07.magtech.org.cn/yxlcyj/EN/10.3969/j.issn.1671-7171.2025.10.023 OR http://journal07.magtech.org.cn/yxlcyj/EN/Y2025/V42/I10/1760

[1] DEZERN A E, ZAHURAK M L, SYMONS H J, et al. Haploidentical BMT for severe aplastic anemia with intensive GVHD prophylaxis including posttransplant cyclophosphamide[J].Blood Adv,2020, 4(8):1770-1779.
[2] KIM H, LEE K H, SOHN S K, et al. Effect of stem cell source and dose on allogeneic hematopoietic stem cell transplantation in adult patients with idiopathic aplastic anemia: Data from the Korean aplastic anemia trials[J].Acta Haematol,2020, 143(3):232-243.
[3] SHALLIS R M, AHMAD R, ZEIDAN A M. Aplastic anemia: Etiology, molecular pathogenesis, and emerging concepts[J].Eur J Haematol,2018, 101(6):711-720.
[4] SHAH S, JAIN P, SHAH K, et al. Immunosuppressive therapy for aplastic anemia: a single-center experience from western India[J].Ann Hematol,2019, 98(1):41-46.
[5] 王文婷, 顾顺利, 陈要臻, 等. 微柱凝胶卡交叉配血法导致血型抗体漏检的探讨[J].中国输血杂志, 2019, 32(7):81-83.
[6] 王晓东, 王然, 陈盟,等. 基于梯度密度离心原理的干式全血分析系统设计[J].军事医学, 2019, 43(9):50-56.
[7] 中华医学会血液学分会红细胞疾病(贫血)学组. 再生障碍性贫血诊断与治疗中国专家共识(2017年版)[J].中华血液学杂志, 2017, 38(1):1-5.
[8] GAMBINO C M, DI BONA D, AIELLO A, et al. HLA-C1 ligands are associated with increased susceptibility to systemic lupus erythematosus[J].Hum Immunol,2018, 79(3):172.
[9] HSU K, KUO M S, YAO C C, et al. The MNS glycophorin variant GP.Mur affects differential erythroid expression of Rh/RhAG transcripts[J].Vox Sang,2017, 112(7):671-677.
[10] SHEHI E, GHAZANFAR H, FORTUZI K, et al. A rare case of parvovirus B19 infection manifesting as chronic aplastic anemia and neutropenia in a human immunodeficiency virus-infected patient[J].Cureus,2020, 12(12):e12174.
[11] 侯瑞琴, 杨洪燕, 崔银平, 等. 患者不规则抗体鉴定结果分析及其临床意义[J].中国实验血液学杂志, 2020, 28(3):257-262.
[12] GASSNER C, DEGENHARDT F, MEYER S, et al. Low-frequency blood group antigens in Switzerland[J].Transfus Med Hemother,2018, 45(4):239-250.
[13] 贾小村. 红细胞悬液与血浆不同比例输注在异位妊娠破裂大出血患者大量输血中的应用效果观察[J].实验与检验医学,2020,38(2):411-414.
[14] SCHOETTLER M L, NATHAN D G. The pathophysiology of acquired aplastic anemia:current concepts revisited[J].Hematol Oncol Clin North Am,2018, 32(4):581-594.

[1]	. [J]. JOURNAL OF CLINICAL RESEARCH, 2025, 42(9): 1639-1641.