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| Diagnostic Value of Free Antibody Test and Direct Antiglobulin Test in Hemolytic Disease of the Newborn |
| QIU Cui, LI Ya, LI Xiaolei |
| Department of Blood Transfusion, Puyang People's Hospital, Puyang Henan 457000 |
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Abstract 【Objective】To explore the diagnostic value of the free antibody test and direct antiglobulin test (DAT) in hemolytic disease of the newborn (HDN).【Methods】 Blood samples were collected from 200 suspected HDN infants and their mothers for free antibody test and DAT. The incidence of HDN was compared across different genders, blood types, maternal-fetal blood type combinations, and antibody titers. The relationship between infant age (counted by days) and the results of free antibody test and DAT was analyzed. The diagnostic efficacy of the combined detection of free antibody test and DAT was evaluated using the receiver operating characteristic (ROC) curve.【Results】 Among 200 suspected HDN cases, 108 were diagnosed with HDN. There was no statistically significant difference in the incidence of HDN between male and female infants (P>0.05). The incidence of ABO HDN was significantly higher than that of Rh HDN (P<0.05). The incidence of O-A type (indicating maternal and infant blood type) HDN was higher than that of O-B type HDN (P<0.05). The incidence of HDN increased with rising antibody titers (<64, 64, 128, 256), and the differences among the groups were statistically significant (P<0.05). The detection rate of HDN by the free antibody test was higher than that of DAT. The effect of infant age on the free antibody test was not statistically significant (P>0.05), whereas it had a significant impact on DAT (P<0.05). The ROC curve analysis showed that the area under the curve for the combined detection of free antibody test and DAT was significantly larger than that for either test alone (P<0.05).【Conclusion】 The free antibody test and DAT have certain diagnostic value for HDN, and their combined detection provides a higher diagnostic value. It is recommended for widespread application.
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Received: 02 December 2024
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2025, Vol. 42 
