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| Reversal of Cisplatin Resistance in Ovarian Cancer SKOV3/cDDP Cell Line by Mesothelin Silence |
| ZHENG Fu-li, YANG Yong-kang, LIU Ya-hong, et al |
| Department of Obstetrics, The Second Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine, Xi'an Shaanxi 712000,China |
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Abstract 【Objective】To investigate the effects and mechanisms of mesothelin silencing (MSLN) on reversing the cisplatin resistance of ovarian cancer drug cisplatin-resistant SKOV3/DDP cell line. 【Methods】The expression level of MSLN mRNA and MSLN protein in SKOV3/cDDP cells and SKOV3 cells were detected by fluorescence quantitative PCR and Western blotting, respectively. SKOV3/cDDP cells were transfected with mesothelin RNAi lentivirus solution and empty vector lentivirus solution, respectively, to establish a stable cell line SKOV3/cDDP-LV-MSLN-RNAi (the experimental group), SKOV3/DDP-MSLN-neg (the negative control group). The sensitivity of the SKOV3/cDDP-LV-MSLN-RNAi cells to cDDP was detected by MTT assay. The apoptosis rate of SKOV3/cDDP-LV-MSLN-RNAi cells was examined by flow cytometry (FCM). 【Results】The expression level of MSLN mRNA and MSLN protein in SKOV3/cDDP cells were significantly higher than that in sensitive SKOV3 cells(P<0.01). The expression of MSLN protein in cells with transfected mesothelin RNAi lentivirus solution was significantly lower than that in cells with transfected empty vector lentivirus solution and cells without any transfection .The difference was statistically significant (P<0.01). The half inhibition concentration (IC50) of the cells in the experimental group was significantly lower than that in the negative control group and the blank group (P<0.01). The apoptotic rate of SKOV3/cDDP-LV-MSLN-RNAi cells in the experimental group was significantly higher than that in the negative control group and the blank group, and the difference was statistically significant. 【Conclusion】MSLN silence can enhance the sensitivity of ovarian cancer SKOV3/cDDP cells to cDDP, promote the apoptosis of drug-resistant ovarian cancer cells induced by cDDP, and to a certain degree reverse the cisplatin resistance of ovarian cancer SKOV3/cDDP cell lines.
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Received: 10 December 2019
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2021, Vol. 38 
