呼吸道合胞病毒肺炎患儿Th1、Th2细胞及其分泌的细胞因子与肺功能的相关性分析

doi:10.3969/j.issn.1671-7171.2021.10.026

Abstract
Figure/Table
References (0)
Related Citation (15)

Download: PDF (1185 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract 【Objective】To investigate the correlation between helper T cells (Th) 1, Th2 cells, cytokines and lung function in children with respiratory syncytial virus (RSV) pneumonia. 【Methods】A total 80 children with respiratory syncytial virus pneumonia treated in our hospital from November 2017 to December 2020 were selected as RSV group, and 52 infants who underwent physical examination in our hospital at the same time were selected as control group. The percentages of Th1 and Th2 cells in peripheral blood of the two groups were detected by flow cytometry, and Th1/Th2 were calculated; the cytokine interferon (IFN-γ) and interleukin (IL) - 4 levels secreted by Th1 and Th2 cells was detected by enzyme-linked immunosorbent assay (ELISA) ; the pulmonary function of the two groups was detected by pulmonary function tester, and the Th1/Th2 and serum IFN-γ,IL-4 level and pulmonary function index were compared between the two groups; Pearson was used to analyze the correlation between Th1/Th2 ,serum IFN-γ, IL-4 level and pulmonary function indexes. 【Results】 There was no significant difference in gender, age and body mass index (BMI) between the two groups (P>0.05); Percentage of Th1 cells, Th1/Th2 and IFN-γ in peripheral blood of the RSV group. The percentage of Th2 cells and IL-4 in peripheral blood were significantly higher than those in the control group (P<0.05); Minute ventilation (MV), tidal volume (VT), inspiratory time (TI), expiratory time (TE), respiratory ratio (Ti/TE) and peak expiratory flow rate (PEF) in the RSV group were significantly lower than those in the control group, and respiratory rate (RR) was significantly higher than those in the control group (P<0.05); Th1/Th2 was positively correlated with MV, VT, TE and PEF (r=0.86, 0.57, 0.34, 0.67, P<0.05), and negatively correlated with RR (r=-0.69, P<0.05); IFN-γ was positively correlated with VT, Ti/TE and PEF (r=0.47, 0.64, 0.70, P<0.05), and negatively correlated with RR (r=-0.81, P<0.05); IL-4 was negatively correlated with MV, VT, Ti/TE and PEF (r=-0.47, -0.60, -0.32, -0.63, P<0.05). 【Conclusion】 Th2 dominant response is the main manifestation of immune response in children with RSV pneumonia. Th1, Th2 cells and their secreted cytokines are significantly correlated with pulmonary function indexes, which can be used as indexes for judging the condition and evaluating the prognosis.

Key words： Respiratory Syncytial Viruses Pneumonia, Viral Th1 Cells/IM Th2 Cells/IM Respiratory Function Tests Child

Received: 20 August 2021

PACS:

R725.631.2

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LEI Rong
	SHEN Jie

Cite this article:

LEI Rong,SHEN Jie. Correlation between Th1, Th2 Cells, Cytokines and Pulmonary Function in Children with Respiratory Syncytial Virus Pneumonia[J]. JOURNAL OF CLINICAL RESEARCH, 2021, 38(10): 1533-1536.

URL:

http://journal07.magtech.org.cn/yxlcyj/EN/10.3969/j.issn.1671-7171.2021.10.026 OR http://journal07.magtech.org.cn/yxlcyj/EN/Y2021/V38/I10/1533

[1] 徐淑静,刘新泳,展鹏. 呼吸道合胞病毒抑制剂研究新进展[J].药学学报,2020,55(4):597-610.
[2] BAWAGE S S, TIWARI P M, PILLAI S, et al. Antibiotic minocycline prevents respiratory syncytial virus infection[J].Viruses,2019,11(8):739.
[3] SHIBATA T, MAKINO A, OGATA R, et al. Respiratory syncytial virus infection exacerbates pneumococcal pneumonia via Gas6/Axl-mediated macrophage polarization[J].J Clin Invest,2020,130(6):3021-3037.
[4] REY-JURADO E, KALERGIS A M. Immunological features of respiratory syncytial virus-caused pneumonia-implications for vaccine design[J].Int J Mol Sci,2017,18(3):556.
[5] HUANG C, ZHANG Z, WANG L, et al. ML-7 attenuates airway inflammation and remodeling via inhibiting the secretion of Th2 cytokines in mice model of asthma[J].Mol Med Rep,2018,17(5):6293-6300.
[6] 郑海涛,任献青,闫永彬,等. 人呼吸道合胞病毒感染对机体Th1、Th2影响的研究进展[J].病毒学报,2020,36(4):719-725.
[7] 梁婷婷,王丹晶. 呼吸道合胞病毒肺炎新生儿血清炎性因子水平变化及与肺功能的相关性[J].中国妇幼保健,2020,35(2):246-248.
[8] 胡亚美, 江载芳, 诸福棠.实用儿科学[M].第7版.北京:人民卫生出版社, 2002:1180.
[9] 龚成,王雪,罗明,等. 重症呼吸道合胞病毒肺炎的危险因素分析[J].疾病监测,2020,35(7):613-617.
[10] 杜春燕,王春辉. 不同剂量重组人干扰素α2b注射液雾化吸入治疗小儿呼吸道合胞病毒肺炎疗效比较[J].陕西医学杂志,2020,49(1):105-108.
[11] 杨嫚,李文莲,刘婷婷,等. 呼吸道合胞病毒感染对百日咳患儿T淋巴细胞亚群、肺功能及炎症因子的影响[J].中华医院感染学杂志,2020,30(20):3155-3159.
[12] 麦朗君,翟佳羽,林烈桔,等. 呼吸道合胞病毒下呼吸道感染患儿外周血miR-145表达及与Th1/Th2平衡的关系[J].河北医学,2020,26(8):1277-1281.
[13] FANG D, CUI K, HU G, et al. Bcl11b, a novel GATA3-interacting protein, suppresses Th1 while limiting Th2 cell differentiation[J].J Exp Med,2018,215(5):1449-1462.
[14] FEDELE G, SCHIAVONI I, NENNA R, et al. Analysis of the immune response in infants hospitalized with viral bronchiolitis shows different Th1/Th2 profiles associated with respiratory syncytial virus and human rhinovirus[J].Pediatr Allergy Immunol,2018,29(5):555-557.
[15] 代丽,王薇,舒俊华,等. 婴幼儿呼吸道合胞病毒肺炎潮气呼吸肺功能变化[J].中华医院感染学杂志,2020,30(5):757-761.
[16] 白东琴,黄红艳,王雪红. 呼吸道合胞病毒肺炎患儿NK细胞与肺功能关系研究[J].西南国防医药,2021,31(1):4-7.