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| Expression and Correlation of NF-κB with Glucocorticoid Receptor of Oral Mucosa in Patients with Oral Lichen Planus |
| YANG Xiu-chuan, LI Fang, SUN Yan |
| Department of Stomatology, Anting Hospital,Shanghai Jiading District, Shanghai 201805 |
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Abstract 【Objective】 To study the expression and correlation of nuclear factor-κ B (NF-κ b) with glucocorticoid receptor (GR) of oral mucosa in patients with oral lichen planus (OLP). 【Methods】 A total of 126 patients with oral lichen planus who were treated in Department of Stomatology, Anting Hospital,Shanghai Jiading District from February 2017 to February 2019 were selected as clinical research objects (the observation group) for retrospective analysis. And 60 healthy oral mucosa extracted from the oral mucosa, which required to be removed for extraction of impacted teeth in outpatient department, were selected as the control group. The expression of the subunits of NF-κ b including P50 and p65, and the subtypes of GR including GR β (glucocorticoid receptor β) and GR α (glucocorticoid receptor α) in the mucosa of the two groups were analyzed by RT-PCR and Western blotting, and the correlation between NF-κ B and GR in OLP was explored. 【Results】 There were lesions in gingiva, tongue, buccal and other areas in the observation group. The main types of lesions were erosive and mottled. The main pathological manifestations were: banded infiltration of lymphocytes in lamina propria, disorder of basal cell arrangement, irregular prolongation of epithelial nail process, acanthosis, epithelial hyperkeratosis. The expressions of NF-κ B P50, p65, GR β and GR α were shown in the oral mucosa of the two groups. Among them, the expression of GR α protein in the observation group decreased, while the expression of P50 and p65 increased. However, there was no significant change in GR β. The expression level of GR α mRNA in the observation group was significantly lower than that in the control group, the difference was statistically significant (P<0.05); the expression level of P50 and p65 in the observation group was significantly higher than that in the control group, the difference was statistically significant (P<0.05); there was no significant difference in GR β mRNA level between the two groups, the difference was not statistically significant (P>0.05). In the mucosa of patients with oral lichen planus, the expression of P50 was negatively correlated with GR α (r=-0.536,P=0.002). There was also a negative correlation between p65 and GR α (r=-0.428,P=0.015). 【Conclusion】 Under the influence of the clinical treatment effect of glucocorticoids and the chronic inflammation of oral lichen planus, the GR α level of the patients with this disease is down-regulated. NF-κ B and GR have antagonistic effect in the inflammatory response of this disease.
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Received: 05 January 2021
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2021, Vol. 38 
