|
|
|
| Effects and Novel Mechanism of Emodin on Cytochrome C, bcl-2, AKT and ERK in Intestinal Epithelial Inflammatory Model Caco-2 Cells |
| XU Xian-feng, ZHANG lin |
| ChangSha City Hospital of Traditional Chinese Medicine, Changsha Hunan 410100 China |
|
|
|
|
Abstract 【Objective】To explore the effects of emodin on the expression of cytochrome C, bcl-2, PI3-K/Akt and ERK signaling pathways in inflammatory model of intestinal epithelial cell Caco-2.【Methods】A lipopolysaccharide (LPS)-induced Caco-2 inflammatory cell model was established and treated with different concentrations of emodin. The apoptosis, phosphorylation of Akt and ERK, cytochrome C and bcl-2 protein expression in Caco-2 cells before and after different concentrations of emodin treatment were compared.【Results】After induction of Caco-2 cells by LPS for 24 h, the apoptosis of Caco-2 cells was increased compared to vehicle control cells. Meanwhile the expression of cytochrome C was increased, and the expression of bcl-2 protein was decreased. After treatment with different concentrations of emodin for 24 h in LPS-induced Caco-2 cells, cell apoptosis was inhibited, cytochrome C expression decreased with increasing emodin concentration, and bcl-2 protein expression increased. Pretreatment with phosphatidylinositol kinase (PI3K) inhibitor LY294002 and extracellular signal-regulated kinase (ERK1/2) PD98059 reversed the inhibition of apoptosis by emodin, while increasing cytochrome C expression and decreasing the expression of bcl-2 as well. Also, emodin treatment inhibited the phosphorylation of Akt and ERK.【Conclusion】In the inflammatory model of intestinal epithelial cell line Caco-2, emodin inhibits cell apoptosis by regulating PI3-K/Akt and ERK signaling pathways through inhibiting phosphorylation of Akt and ERK. Emodin also reduced the expression of apoptotic cytochrome C and enhanced the expression of bcl-2.
|
|
Received: 23 August 2017
|
|
|
|
|
|
[1] 王晓娣. 炎症性肠病患者肠黏膜组织细胞凋亡[J].世界华人消化杂志, 2008, 16(28): 3197-3199.
[2] 王明华,曹永清. 复方大黄粉治疗溃疡性结肠炎临床疗效观察[J].辽宁中医学院学报,2005,7(3):195-196.
[3] Elmore S. Apoptosis: a review of programmed cell death[J].Toxicol Pathol,2007,35(4):495-516.
[4] Lee HZ. Effects and mechanisms of emodin on cell death in human Lung squamous cell carcinoma[J].Br J Pharmacol,2001, 134(1):11-20.
[5] 丁博文, 嵇武, 白小武,等.大黄素对肠上皮细胞损伤的保护作用及机制研究[J].东南国防医药, 2012,14(3):199-202.
[6] Wei J, Feng J. Signaling pathways associated with inflammatory bowel disease[J].Recent Pat Inflamm Allergy Drug Discov,2010,4(2):105-117.
[7] Viscomi MT, Oddi S, Latini L,et al.Selective CB2 receptor agonism protects central neurons from remote axotomy-induced apoptosis through the PI3K/Akt pathway[J].J Neurosci,2009,29(14):4564-4570. |
|
|
|
2019, Vol. 36 
