|
|
|
| Regulatory Effect of Maizhuni on Rabbit Arthritic Chondrocyte Apoptosis |
| MIAO De-sheng, WANG Yun, CHENG Guo-jie , et al |
| Traditional Chinese Medicine Institute of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, 830000 China |
|
|
|
|
Abstract Objective To explore the regulation effect and mechanism of Maizhuni on the apoptosis of rabbit chondrocytes of arthritis. Methods Apoptosis of arthritic chondrocytes was induced by IL-1beta. Normal serum or low, medium and high dose of Maizhuni serum were added to culture medium to regulate apoptosis of arthritic chondrocytes. Cell viability was measured by MTT assay. Cell apoptosis was detected by flow cytometry using the Annexin V-FITC/PI double staining. The expression level of Fas, FasL, caspase-8, caspase-3, I-FLICE, PARP was analyzed by real time quantitative PCR and western blotting. Results Compared to normal serum mimic group, all doses of maizhuni-treated groups significantly increased the viability of chondrocytes (P<0.001) and significantly reduced early apoptosis of chondrocytes (P<0.05). Meanwhile, maizhuni significantly decreased the expression levels (mRNA and protein) of Fas, FasL, caspase-8 and caspase-3, while increased the expression of I-FLICE and PARP (P<0.001). Conclusion Maizhuni inhibits the apoptosis of rabbit chondrocytes induced by IL-1 beta through mechanism of regulating Fas/FasL signal pathway.
|
|
Received: 05 February 2018
|
|
|
|
|
|
[1] Kim HT, Lo MY, Pillarisetty R. Chondrocyte apoptosis following intraarticular fracture in humans[J].Osteoarthritis Cartilage,2002, 10(9): 747-749.
[2] 孙国梁,吕发明.膝关节骨性关节炎危险因素的病例对照研究[J].新疆中医药,2008,26 (3):44-45.
[3] 袁国祥, 卢勇, 孟庆才, 等. 关节镜清理术配合维药治疗膝骨性关节炎20例[J].河南中医, 2011, 31(12):1406-1407.
[4] 洪汉刚, 张耀武, 牛清海, 等. 维药买朱尼对关节软骨前炎性因子的影响[J].中国组织工程研究, 2013, 17(7):1162-1167.
[5] 孟庆才, 方锐, 王拥军, 等. 维药买朱尼对膝骨性关节炎大鼠关节软骨中 Fas 及 FasL mRNA 表达的调节效应[J].中国临床康复, 2006, 10(35): 31-33.
[6] 肖晟, 李康华, 吕红斌, 等. 川芎嗪对家兔软骨细胞 Bcl-2 蛋白表达及凋亡的影响[J].湖南医科大学学报, 2003, 28(3): 224-226.
[7] 黄松, 陈敬有. 黄芩素对IL-1β诱导的软骨细胞炎症反应的影响[J].医学临床研究, 2017, 34(7): 1332-1334.
[8] Ding Q, Zhong H, Qi Y,et al.Anti-arthritic effects of crocin in interleukin-1β-treated articular chondrocytes and cartilage in a rabbit osteoarthritic model[J].Inflamm Res,2013, 62(1): 17-25.
[9] Blanco FJ, Guitian R, qazquez-Martul E,et al.Osteoarttis chondroeytesdie by apoptosis.A possible Pathway for osteoartis Pathology[J].Arthritis Rheum,1998,41(2):284-289.
[10] 孙辉平, 杨金凤, 邹双发, 等. 瑞芬太尼预处理对鼠肝缺血再灌注损伤时肾细胞凋亡及 Bcl-2, caspase-3 的影响[J].医学临床研究, 2017, 34(6): 1115-1118. |
| [1] |
. [J]. JOURNAL OF CLINICAL RESEARCH, 2019, 36(4): 740-741. |
|
|
|
|
2019, Vol. 36 
