Abstract:【Objective】 To explore the application effect of operating room intervention guided by the Enhanced Recovery After Surgery(ERAS) concept in patients undergoing laparoscopic cholecystectomy. 【Methods】 A total of 100 patients with gallstones who underwent laparoscopic cholecystectomy in our hospital from January 2022 to January 2024 were selected. The patients were divided into the observation group and the control group by random number table method, with 50 cases in each group. The observation group was given operating room intervention guided by the ERAS concept, while the control group was given routine nursing intervention. The body temperature of the two groups was compared at 30 minutes after anesthesia(T1), 1 hour after anesthesia(T2), after entering the post-anesthesia care unit(PACU)(T3), and when leaving the PACU(T4). The Visual Analogue Scale(VAS) scores at 6 hours, 12 hours, 24 hours, and 72 hours after surgery, recovery time indicators, social function [Social Disability Screening Schedule(SDSS) score], psychological stress [Hamilton Anxiety Scale(HAMA) score, Hamilton Depression Scale(HAMD) score], and quality of life [World Health Organization Quality of Life Scale(WHO-QOL) score] were compared between the two groups. 【Results】 The body temperature of the observation group at T2, T3, and T4 was higher than that of the control group, with statistically significant differences(P<0.05); the VAS scores of the observation group at 12 hours, 24 hours, and 72 hours after surgery were lower than those of the control group, with statistically significant differences(P<0.05). The time of endotracheal tube extubation, stay time in PACU, first ambulation time, first anal exhaust time, resumption of eating time, and hospitalization time of the observation group were shorter than those of the control group, with statistically significant differences(P<0.05). After intervention, the SDSS, HAMA, and HAMD scores of both groups were lower than those before intervention, and the observation group was lower than the control group, with statistically significant differences(P<0.05). After intervention, the scores of social relations, physical function, environmental dimension, psychological function and total score of both groups were higher than those before intervention, and the observation group was higher than the control group, with statistically significant differences(P<0.05). 【Conclusion】 The operating room intervention guided by the ERAS concept has a good application effect in patients undergoing laparoscopic cholecystectomy. It can effectively reduce the fluctuation range of perioperative body temperature, alleviate postoperative pain, shorten the recovery time, improve social function and psychological stress state, and enhance the quality of life, which is worthy of promotion.