医学临床研究
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医学临床研究  2021, Vol. 38 Issue (4): 493-496    DOI: 10.3969/j.issn.1671-7171.2021.04.004
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核因子κB与糖皮质激素受体在口腔扁平苔藓患者口腔黏膜组织中的表达及其意义*
杨绣川1, 黎芳1, 孙燕2**
1.上海市嘉定区安亭医院口腔科,上海 201805;
2.上海交通大学医学院附属第九人民医院口腔科,上海 200011
Expression and Correlation of NF-κB with Glucocorticoid Receptor of Oral Mucosa in Patients with Oral Lichen Planus
YANG Xiu-chuan, LI Fang, SUN Yan
Department of Stomatology, Anting Hospital,Shanghai Jiading District, Shanghai 201805
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摘要 【目的】 探讨核因子κB(nuclear factor-κB,NF-κB)、糖皮质激素受体(glucocorticoid receptor,GR)在口腔扁平苔藓(oral lichen planus,OLP)患者口腔黏膜组织中的表达及其意义。【方法】 选取2017年2月至2019年2月在上海市嘉定区安亭医院诊治的口腔扁平苔藓患者局部病损口腔黏膜标本126例(观察组),另外选取在上海市嘉定区安亭医院门诊行阻生牙拔除术需切除的健康口腔黏膜(60例),采用实时定量反转录PCR(reverse transcription-PCR,RT-PCR)与蛋白质印迹法检测两组患者黏膜中NF-κB的亚型p50、p65以及GR的亚型糖皮质激素受体β(GRβ)、糖皮质激素受体α(GRα)表达水平,并分析NF-κB与GR的相关性。【结果】 观察组在牙龈、舌、颊等区域黏膜均有病损,主要损伤类型包括糜烂型以及斑纹型;病理表现:固有层淋巴细胞带状浸润,基底细胞排列紊乱,上皮钉突不规则延长,棘层增生,上皮不全角化。两组口腔黏膜中p50、p65、GRβ、GRα均有表达,其中观察组中GRα蛋白表达减少,p50、p65表达增加,GRβ无显著变化。观察组中GRα mRNA表达水平显著低于对照组,差异具有统计学意义(P<0.05);观察组中p50、p65表达水平显著高于对照组的,差异具有统计学意义(P<0.05),但两组中GRβ mRNA水平比较差异无统计学意义(P>0.05)。在口腔扁平苔藓患者黏膜中,p50、p65与GRα均呈负相关(r=-0.536,P=0.002 ;r=-0.428,P=0.015)。【结论】 口腔扁平苔藓患者的GRα水平降低,p50、p65表达增加,在该疾病的炎症反应中NF-κB与GR有拮抗作用。
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杨绣川
黎芳
孙燕
关键词 扁平苔癣,口腔/病理学NF-κB受体,糖皮质激素口腔黏膜    
Abstract【Objective】 To study the expression and correlation of nuclear factor-κ B (NF-κ b) with glucocorticoid receptor (GR) of oral mucosa in patients with oral lichen planus (OLP). 【Methods】 A total of 126 patients with oral lichen planus who were treated in Department of Stomatology, Anting Hospital,Shanghai Jiading District from February 2017 to February 2019 were selected as clinical research objects (the observation group) for retrospective analysis. And 60 healthy oral mucosa extracted from the oral mucosa, which required to be removed for extraction of impacted teeth in outpatient department, were selected as the control group. The expression of the subunits of NF-κ b including P50 and p65, and the subtypes of GR including GR β (glucocorticoid receptor β) and GR α (glucocorticoid receptor α) in the mucosa of the two groups were analyzed by RT-PCR and Western blotting, and the correlation between NF-κ B and GR in OLP was explored. 【Results】 There were lesions in gingiva, tongue, buccal and other areas in the observation group. The main types of lesions were erosive and mottled. The main pathological manifestations were: banded infiltration of lymphocytes in lamina propria, disorder of basal cell arrangement, irregular prolongation of epithelial nail process, acanthosis, epithelial hyperkeratosis. The expressions of NF-κ B P50, p65, GR β and GR α were shown in the oral mucosa of the two groups. Among them, the expression of GR α protein in the observation group decreased, while the expression of P50 and p65 increased. However, there was no significant change in GR β. The expression level of GR α mRNA in the observation group was significantly lower than that in the control group, the difference was statistically significant (P<0.05); the expression level of P50 and p65 in the observation group was significantly higher than that in the control group, the difference was statistically significant (P<0.05); there was no significant difference in GR β mRNA level between the two groups, the difference was not statistically significant (P>0.05). In the mucosa of patients with oral lichen planus, the expression of P50 was negatively correlated with GR α (r=-0.536,P=0.002). There was also a negative correlation between p65 and GR α (r=-0.428,P=0.015). 【Conclusion】 Under the influence of the clinical treatment effect of glucocorticoids and the chronic inflammation of oral lichen planus, the GR α level of the patients with this disease is down-regulated. NF-κ B and GR have antagonistic effect in the inflammatory response of this disease.
Key wordsLichen Planus, Oral/PA    NF-kappa B    Receptors, Glucocorticoid    Mouth Mucosa
收稿日期: 2021-01-05     
中图分类号:  R781.59  
基金资助:* 上海市卫生和计划生育委员会科研课题(编号:20184Y0189)
通讯作者: ** E-mail: sunyan18@126.com   
引用本文:   
杨绣川, 黎芳, 孙燕. 核因子κB与糖皮质激素受体在口腔扁平苔藓患者口腔黏膜组织中的表达及其意义*[J]. 医学临床研究, 2021, 38(4): 493-496.
YANG Xiu-chuan, LI Fang, SUN Yan. Expression and Correlation of NF-κB with Glucocorticoid Receptor of Oral Mucosa in Patients with Oral Lichen Planus. JOURNAL OF CLINICAL RESEARCH, 2021, 38(4): 493-496.
链接本文:  
http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2021.04.004     或     http://journal07.magtech.org.cn/yxlcyj/CN/Y2021/V38/I4/493
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