Abstract：【Objective】To investigate the expression of CTHRC1 (collagen triple helix repeat containing 1),TGF-β1 and smad7 in OSF (Oral Submucous Fibrosis) by immunohistochemistry in order to explore the correlation between CTHRC1 and TGF-β1/smad signaling pathway in pathogenesis of OSF. 【Methods】Buccal mucous tissues were obtained from 33 OSF patients (10 cases in the early stage, 13 cases in the middle stage and 10 cases in the late stage) and 10 healthy volunteers' mucous tissues. After paraffin embedding, the expression of CTHRC1,TGF-β1 and smad7 proteins in sections were detected by immunohistochemistry. The correlations between TGF-β1 expression and CTHRC1, smad7 were tested by Pearson software. 【Results】The expression of CTHRC1 in the early, middle and later stage OSF was significantly higher than that in normal oral mucosa(P=0.007,0.002,0.006). SO did the expression of smad7 in all stages of OSF(P=0.032,0.007,0.005). The expression of TGF-β1 was increased in the early and middle stage OSF tissues (P=0.007,0.031), however, there was no significant difference between the late expression and the normal control (P=0.323). There was a positive correlation between TGF-β1 and CTHRC1 expression in OSF lesions (R=0.678,P=0.0010), and there was no significant correlation between TGF-β1 and Smad7 expression (R=0.003,P=0.9908). 【Conclusions】The expression of CTHRC1 increases significantly in all stages of OSF,which is positively correlated with the expression of TGF-β1. CTHRC1 can be a new target for anti-fibrosis.
[1] 李霞.肌成纤维细胞在口腔黏膜下纤维性变发病机制中的作用研究[D].中南大学,2006. [2] 谢辉,凌天牖,郭金陵,等.口腔黏膜下纤维性变发病相关基因的功能分析[J].口腔医学研究,2007,23(2):128-131. [3] 谢辉,刘健,凌天牖.细胞因子IL-6,TGF-β1在口腔黏膜下纤维性变中的表达[J].临床口腔医学杂志,2012,28(10):592-594. [4] Nakao A, Okumura K, Ogawa H. Smad7: a new key player in TGF-beta-associated disease[J].Trends Mol Med,2002,8(8):361-363. [5] Choy L, Skillington J, Derynck R. Roles of autocrine TGF-beta receptor and Smad signaling in adipocyte differentiation[J].J Cell Biol,2000,149(3):667-682. [6] Nakao A, Afrakhte M, Morén A,et al.Identification of Smad7, a TGFbeta-inducible antagonist of TGF-beta signalling[J].Nature,1997,389(6651):631-635. [7] 蔺琳,凌天牖.口腔黏膜下纤维化中结缔组织生长因子表达的研究[J].临床口腔医学杂志,2015,31(3):152-154.
2019, Vol. 36 
