Abstract：【Objective】 To establish a Neisseria gonorrhoeae (NG) nucleic acid rapid test method and assess the analytic sensitivity and specificity and detection consistency with quantitative PCR method. 【Methods】 The rapid detection method of Neisseria gonorrhoeae nucleic acid was established by RPA-LFA; the sensitivity and specificity of the method were evaluated by using the national reference for Neisseria gonorrhoeae nucleic acid detection; sixty-one samples of clinical genital secretions were detected by fluorescence quantitative PCR kit and RPA-LFA respectively, and the consistency of the detection results was evaluated.【Results】 The sensitivity of RPA-LFA method was 104 cells/mL, the specificity was good, 10 different Neisseria gonorrhoeae strains could be detected, 10 non Neisseria gonorrhoeae strains could not be detected. The consistency between RPA-LFA method and fluorescent quantitative PCR method was high (Kappa=0.97), the positive detection rate of RPA-LFA method was 52.5% (32/61) and that of fluorescent quantitative PCR method was 54.1% (33/61). The sensitivity and specificity of rpa-lfa were 96.97% and 100.00% respectively, and the flow time of real-time PCR for single sample was 120±20min, significantly longer than 35±5min of RPA-LFA (P<0.05).【Conclusion】 The RPA-LFA method for rapid detection of Neisseria gonorrhoeae nucleic acid is successfully established. The method has high consistency with the fluorescent quantitative PCR method, and does not need complex and precise temperature control equipment. The interpretation of detection results is simple, which shortens the detection time of Neisseria gonorrhoeae nucleic acid and reduces the cost.
王志宇, 屠博文, 陈红岩. 一种淋球菌核酸快速检测方法的建立与评价[J]. 医学临床研究, 2020, 37(7): 982-985.
WANG Zhi-yu, TU Bo-wen, CHEN Hong-yan. Establishment and Evaluation of a Rapid Detection Method for Neisseria Gonorrhoeae Nucleic Acid. JOURNAL OF CLINICAL RESEARCH, 2020, 37(7): 982-985.
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2020, Vol. 37 
