Abstract：【Objective】To study the effect of hyaluronic acid (HA) mediated nano silver targeted liposomes on burn wound healing, and to explore the ratio of HA to Ag sulfadiazine.【Methods】The formulation includes 100% sulfadiazine silver (control formulation), 95% sulfadiazine silver + 5% hyaluronic acid (formulation a), 90% sulfadiazine silver + 10% hyaluronic acid (formulation b). 54 SD rats with deep second degree burn were randomly divided into control group, experimental group A and experimental group B, with 18 rats in each group. Six rats in each group were killed on d7 and d14 after the application of control formula, formula A and formula B respectively. The changes of hydroxyproline, collagen ratio and DNA proliferation cycle of dermal cells were measured.The time of wound healing and the area of residual wound were observed.【Results】The content of hydroxyproline and the proportion of type Ⅲ/Ⅰ collagen in the wound tissue of group d7 were higher than that of group d3. The content of hydroxyproline and the proportion of type Ⅲ/Ⅰ collagen in the wound tissue of group A were higher than that of group A. the content of hydroxyproline and the proportion of type Ⅲ/Ⅰ collagen in the wound tissue of group B were higher than that of group A (P<0.05). The DNA in G0/G1 phase of dermal cells in d5, d7, d9 and d11 group was significantly lower than that in d3 group, and that in S phase was significantly higher than that in d3 group (P<0.05). The proportion of DNA in G0/G1 phase of d5, d7 and d9 dermal cells in the group A was lower than that in the control group, and the proportion of DNA in G0/G1 phase of d11 dermal cells in the group B was higher than that in the group A; the proportion of DNA in G0/G1 phase of d5 and d7 dermal cells in the group B was lower than that in the group A, and the proportion of DNA in G0/G1 phase of d9 and d11 dermal cells in the group B was higher than that in the group A (P<0.05). The area of residual wound in the group A was lower than that in the control group. The area of residual wound in the group B was lower than that in the group A (P<0.05). The average healing time of the control group was (14.46±1.17) days, higher than that of the experimental group A (12.87±0.75) days, and the average healing time of the experimental group A was higher than that of the experimental group B (11.87± 0.55 days), the difference was statistically significant (P<0.05). 【Conclusion】Hyaluronic acid mediated nano silver targeting liposomes can effectively promote wound healing,and the combination of 90% sulfadiazine and 10% hyaluronic acid is ideal for promoting wound healing.
[1] Dalmedico MM, Meier MJ, Felix JV,et al. Hyaluronic acid covers in burn treatment: a systematic review[J].Rev Esc Enferm USP,2016, 50(3): 522-528. [2] McMahon S, Kennedy R, Duffy P,et al. Poly (ethylene glycol)-based hyperbranched polymer from RAFT and its application as a silver-sulfadiazine-loaded antibacterial hydrogel in wound care[J].ACS Appl Mater Interfaces,2016, 8(40): 26648-26656. [3] 邓忠远. rhGM-CSF联合磺胺嘧啶银治疗深Ⅱ度烧烫伤创面的临床研究[J].医学临床研究, 2017, 34(5): 932-934. [4] Heyneman A, Hoeksema H, Vandekerckhove D, et al. The role of silver sulphadiazine in the conservative treatment of partial thickness burn wounds: A systematic review[J].Burns,2016, 42(7): 1377-1386. [5] 段辉辉, 王瑞. 烧伤创面治疗中透明质酸钠对磺胺嘧啶银药物毒性影响的研究[J].中国美容医学, 2017, 26(2): 65-68. [6] Nimia H H, Carvalho V F, Isaac C, et al. Comparative study of Silver Sulfadiazine with other materials for healing and infection prevention in burns: A systematic review and meta-analysis[J].Burns,2019, 45(2): 282-292. [7] Hedtrich S, Frieβ W. Hyaluronic acid for percutaneous drug delivery//Percutaneous Penetration Enhancers Chemical Methods in Penetration Enhancement[M].Springer, Berlin, Heidelberg,2015: 1-10. [8] Aziz Z, Hassan BAR. The effects of honey compared to silver sulfadiazine for the treatment of burns: a systematic review of randomized controlled trials[J].Burns,2017, 43(1): 50-57. [9] Souza SOL, Cotrim MAP, Oréfice RL, et al. Electrospun poly (ε-caprolactone) matrices containing silver sulfadiazine complexed with β-cyclodextrin as a new pharmaceutical dosage form to wound healing: Preliminary physicochemical and biological evaluation[J].J Mate Sci Mater Med,2018, 29(5): 67. [10] 张磊, 王车江, 刘佳. 湿润烧伤膏与磺胺嘧啶银在深度烧伤保留变性真皮自体皮移植术中的应用比较[J].中国现代医学杂志, 2015, 25(18): 95-97. [11] Mehta MA, Shah S, Ranjan V, et al. Comparative study of silver-sulfadiazine-impregnated collagen dressing versus conventional burn dressings in second-degree burns[J].J Family Med Prim Care,2019, 8(1): 215-219. [12] Ebrahimi-Hosseinzadeh B, Pedram M, Hatamian-Zarmi A, et al. In vivo evaluation of gelatin/hyaluronic acid nanofiber as Burn-wound healing and its comparison with ChitoHeal gel[J].Fibers Polymers,2016, 17(6): 820-826. [13] 蒋瑞明, 张元海, 叶春江, 等. 纳米银烧伤敷料与磺胺嘧啶银乳膏治疗深Ⅱ度烧伤患者创面感染的研究[J].中华医院感染学杂志, 2015, 25(22): 5228-5230.[14] 王斌, 罗旭, 汤从容, 等. 磺胺嘧啶银霜联合重组人粒细胞巨噬细胞刺激因子凝胶治疗深Ⅱ度烧伤创面的疗效评价[J].医药导报, 2015, 34(12): 1588-1590. [15] Cervelli V, Gentile P, Brinci L, et al. Use of platelet rich plasma (PRP) and hyaluronic acid in treatment of extremity gunshot injuries: a case report[J].World J Plast Surg,2016, 5(1): 80-84. [16] 毛庆琳, 韩冬, 付吉东, 等. 银离子抗菌凝胶敷料与磺胺嘧啶银乳膏对女性面部深Ⅱ度烧烫伤创面的处理效果比较[J].山西医药杂志, 2016, 45(3): 308-309. [17] Dumitriu R, Profire L, Nita L, et al. Sulfadiazine—Chitosan Conjugates and Their Polyelectrolyte Complexes with Hyaluronate Destined to the Management of Burn Wounds[J].Materials,2015, 8(1): 317-338. [18] Lucey P, Zouzias C, Franco L, et al. Practice patterns for the prophylaxis and treatment of acute radiation dermatitis in the United States[J].Support Care Cancer,2017, 25(9): 2857-2862.
2019, Vol. 36 
