椎间盘退变期间细胞自噬对MEK、ERK、NRF1、Atg7表达的影响

doi:10.3969/j.issn.1671-7171.2019.07.027

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摘要 【目的】探讨在椎间盘退变期间细胞自噬对丝裂原活化蛋白激酶(MEK)、细胞信号调节激酶(ERK)、转录因子CNC-bZIP蛋白(NRF1)、自噬相关蛋白7(Atg7)表达的影响。【方法】选取腰椎间盘严重变性达到Pfirrmann5级的患者18例(观察组)与年轻腰椎骨折患者18例(对照组)作为研究对象,取腰椎间盘组织分离单个髓核细胞,流式细胞仪分选CD24阳性细胞。在模拟生理压力环境下培养细胞96 h,应用RT-PCR法测定髓核细胞中MEK、ERK、NRF1及Atg7 mRNA的表达,Western blot方法检测髓核细胞中MEK、ERK、NRF1及Atg7蛋白水平的表达。【结果】RT-PCR结果显示:观察组MEK、ERK、NRF1及Atg7 mRNA的表达水平显著高于对照组,差异均有统计学意义(P<0.05);Western blot结果显示:观察组MEK、ERK、NRF1及Atg7蛋白的表达显著高于对照组,差异有显著统计学意义(P<0.05)。【结论】在椎间盘退变期间细胞自噬可上调髓核细胞MEK、ERK、NRF1及Atg7蛋白的表达,MEK、ERK、NRF1、Atg7信号通路是调控髓核细胞凋亡的主要信号传导途径,可能参与腰椎退行性变的发病过程。

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关键词 ：自噬/生理学, 椎间盘/细胞学

Abstract：【Objective】To investigate the autophagy of mitogen-activated protein kinase (MEK), cell signal-regulated kinase (ERK), transcription factor CNC-bZIP protein (NRF1) and autophagy-associated protein 7 (Atg7) during intervertebral disc degeneration to explore the basic research and clinical treatment of lumbar degenerative changes. 【Methods】 A total of 18 patients with severe degeneration of lumbar intervertebral disc (Pfirrmann grade 5) in the observation group and 18 patients with young lumbar vertebrae fractures in the control group were selected as the study subjects. The nucleus pulposus cells were isolated from lumbar intervertebral disc tissue and CD24 positive cells were sorted by flow cytometry. The cells were cultured for 96 h under simulated physiological pressure. The expressions of MEK, ERK, NRF1 and Atg7 mRNA in nucleus pulposus cells were detected by RT-PCR. The levels of MEK, ERK, NRF1 and Atg7 protein in nucleus pulposus cells were detected by Western blot. 【Results】 Results of RT-PCR showed that the expression levels of MEK, ERK, NRF1 and Atg7 mRNA in the observation group were significantly higher than those in the control group t (P<0.05).Western blot results showed that the expression levels of MEK, ERK, NRF1 and Atg7 proteins in the observation group were higher than those in the control group. The differences between the two groups were statistically significant (P<0.05). 【Conclusion】Autophagy upregulates the expression of MEK, ERK, NRF1 and Atg7 proteins in nucleus pulposus cells during degeneration of intervertebral disc. The MEK/ERK/NRF1/Atg7 signaling pathway is the main signaling pathway regulating nucleus pulposus cell apoptosis and may be involved in the pathogenesis of lumbar degenerative changes.

Key words： Autophagy/PH Intervertebral Disc/CY

收稿日期: 2018-11-12

PACS:

R329.25

引用本文:

汪凡栋, 张智, 郑佳状, 陈宇, 廖伟, 刘元彬, 宋昭君. 椎间盘退变期间细胞自噬对MEK、ERK、NRF1、Atg7表达的影响[J]. 医学临床研究, 2019, 36(7): 1326-1328.
WANG Fan-dong, ZHANG Zhi, ZHENG Jia-zhuang,et al. Effect of Autophagy on the Expression of MEK,ERK,NRF1 and Atg7 in Human Nucleus Pulposus Cells during Degeneration of Intervertebral Disc. JOURNAL OF CLINICAL RESEARCH, 2019, 36(7): 1326-1328.

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http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2019.07.027 或 http://journal07.magtech.org.cn/yxlcyj/CN/Y2019/V36/I7/1326

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