大肠埃希菌生物被膜表型、基因型与多重耐药的相关性分析

doi:10.3969/j.issn.1671-7171.2024.05.031

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Abstract 【Objective】To explore the correlation of the bacterial biofilm phenotype and genotype with multidrug resistance in Escherichia coli.【Methods】A total of 230 clinical isolates of Escherichia coli were collected. Genotyping was performed using multiplex PCR, biofilm formation ability was determined using a modified crystal violet assay, and antibiotic susceptibility was tested using the Kirby-Bauer method. Statistical analysis was conducted on the antibiotic resistance patterns of Escherichia coli, the relationship between bacterial biofilm phenotype and multidrug resistance, and the relationship between Escherichia coli genotype and multidrug resistance.【Results】Escherichia coli showed high resistance rates to ampicillin, cefuroxime, and ciprofloxacin, but lower resistance to piperacillin and no resistance to imipenem. Biofilm phenotypes of Escherichia coli were classified as strong, moderate, weak and none, accounting for 3.91%, 31.30%, 57.83% and 6.96%, respectively. The common biofilm phenotypes in Escherichia coli were moderate and weak phenotypes. The stronger biofilm formation ability was associated with higher rate of multidrug resistance. The CTX-M-9 genotype was the most prevalent, accounting for 35.65%. Spearman correlation analysis showed that the biofilm formation ability of Escherichia coli was positively correlated with multidrug resistance rate (P=0.012).【Conclusion】The biofilm formation ability of Escherichia coli is associated with multidrug resistance. Different genotypes exhibit resistance to multiple antibiotics, highlighting the need for strict regulation of antibiotic use in clinical settings.

Key words： Enteropathogenic Escherichia Coli Drug Resistance, Bacterial Bacteria/GE Genotype

Received: 30 June 2023

PACS:

R446.51

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	LU Liquan
	LIU Dan
	XU Sumei

Cite this article:

LU Liquan,LIU Dan,XU Sumei. Analysis of the Correlation of Bacterial Biofilm Phenotype and Genotype with Multidrug Resistance in Escherichia Coli[J]. JOURNAL OF CLINICAL RESEARCH, 2024, 41(5): 754-756.

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http://journal07.magtech.org.cn/yxlcyj/EN/10.3969/j.issn.1671-7171.2024.05.031 OR http://journal07.magtech.org.cn/yxlcyj/EN/Y2024/V41/I5/754

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