CKIP-1对模拟失重诱导的成骨细胞微丝变化的影响<sup>*</sup>

doi:10.3969/j.issn.1671-7171.2024.04.006

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Abstract 【Objective】To investigate the effect of CKIP-1 on microfilament changes induced by simulated weightlessness in osteoblasts.【Methods】We selected mouse osteoblast like cell line (MC3T3-E1) for the study, simulated microgravity environment with a gyrator, and constructed a CKIP-1 overexpression model through lentivirus infection. The cells were divided into group a (microgravity group), group b (empty lentivirus and microgravity group), group c (CKIP-1 overexpression lentivirus and microgravity group), and group d (CKIP-1 overexpression lentivirus group). Fluorescence staining was performed on cells using FITC labeled ghost pen cyclic peptides, and changes in cell microfilaments were observed under laser confocal microscopy. The expression of CKIP-1 mRNA, ALP mRNA, and RUNX2 mRNA was also measured using qRT-PCR technology. 【Results】The microfilament structure of each group of cells showed varying degrees of depolymerization, and the tension fibers were disorderly arranged and the number decreased. The most significant changes were observed in the simulated microgravity group of osteoblasts infected with CKIP-1 overexpression lentivirus. The expression level of CKIP-1 mRNA in the group c and the group d was higher than that in the group a and the group b, and the difference was statistically significant (P<0.05). The expression levels of RUNX2 mRNA and ALPL mRNA in bone cells composed of the group c were lower than those in the group a, the group b, and the group d, with statistical significance(P<0.05). There was no statistically significant difference in the expression levels of RUNX2 mRNA and ALPL mRNA in bone cells between the group a, the group b, and the group d (P>0.05). 【Conclusion】Overexpression of CKIP-1 may further exacerbate the depolymerization of cytoskeletal microfilaments in osteoblasts under microgravity conditions, thereby inhibiting the proliferation and differentiation of osteoblasts.

Key words： Osteoblasts/UL Weightlessness Simulation

Received: 07 February 2023

PACS:

R329.2

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http://journal07.magtech.org.cn/yxlcyj/EN/10.3969/j.issn.1671-7171.2024.04.006 OR http://journal07.magtech.org.cn/yxlcyj/EN/Y2024/V41/I4/500

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