P16/Ki67双染联合HPV基因分型对TCT阴性患者宫颈癌前病变的鉴别价值

doi:10.3969/j.issn.1671-7171.2023.10.004

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Abstract 【Objective】To explore the value of P16/proliferating nuclear antigen (Ki67) double staining combined with human papillomavirus (HPV) genotyping in the identification of cervical precancerous lesions in patients with negative liquid-based thin layer cytology test (TCT). 【Methods】A total of 104 TCT-negative patients who underwent cervical cancer screening in our hospital from May 2021 to July 2022 were selected, all patients underwent P16/Ki67 double staining, HPV genotyping and colposcopy biopsy. The colposcopy biopsy results as the gold standard, the consistency of P16/Ki67 double staining, HPV genotyping and colposcopy biopsy results was analyzed, and the value of P16/Ki67 double staining combined with HPV genotyping in the diagnosis of cervical precancerous lesions in TCT-negative patients was analyzed. 【Results】The results of colposcopy pathological biopsy showed that among the 104 TCT-negative patients, there were 33 patients with cervical intraepithelial neoplasia grade 2 or higher, including 14 patients with CIN2, 18 patients with CIN3, and 1 patient with cervical adenocarcinoma, the remaining 71 cases were all negative lesions. P16/Ki67 double staining was used for the diagnosis of cervical precancerous lesions in TCT-negative patients, 28 positive patients were consistent with the diagnosis results of colposcopy pathological biopsy, and 64 negative patients were consistent with the diagnosis results of colposcopy pathological biopsy, after Kappa consistency test, Kappa=0.738, the agreement between the two diagnostic methods was general. HPV genotyping was used for the diagnosis of cervical precancerous lesions in TCT-negative patients, 25 positive patients were consistent with the diagnosis results of colposcopy biopsy, and 59 negative patients were consistent with the diagnosis results of colposcopy biopsy, after Kappa consistency test, Kappa=0.570. P16/Ki67 double staining combined with HPV genotyping was used for the diagnosis of cervical precancerous lesions in TCT-negative patients, 30 positive patients were consistent with the results of colposcopy biopsy, and 66 negative patients were consistent with colposcopy biopsy, after the Kappa consistency test, Kappa=0.825. The sensitivity, specificity, Youden index and accuracy of P16/Ki67 double staining combined with HPV genotyping were 90.91%, 92.96%, 0.839 and 92.31%, respectively, and the combined diagnosis was more accurate. 【Conclusion】P16/Ki67 double staining combined with HPV genotyping has high diagnostic accuracy for cervical precancerous lesions in TCT-negative patients, and it can be used for early screening of cervical precancerous lesions.

Key words： Cervix Uteri Precancerous Conditions/DI Ki-67 Antigen Genes, p16 Papillomaviridae Genotype

Received: 26 June 2023

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R737.330

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	HAN Shuang
	ZHAI Huali
	LEI Fang
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Cite this article:

HAN Shuang,ZHAI Huali,LEI Fang, et al. Differential Value of P16/Ki67 Double Staining Combined with HPV Genotyping in Cervical Precancerous Lesions in TCT-negative Patients[J]. JOURNAL OF CLINICAL RESEARCH, 2023, 40(10): 1452-1455.

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http://journal07.magtech.org.cn/yxlcyj/EN/10.3969/j.issn.1671-7171.2023.10.004 OR http://journal07.magtech.org.cn/yxlcyj/EN/Y2023/V40/I10/1452

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